LOCUS       sequence-156112-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CSKP. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CSKP_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggccgacg acgacgtgct gttcgaggat gtgtacgagc
      181 tgtgcgaggt gatcggaaag ggtcccttca gtgttgtacg acgatgtatc aacagagaaa
      241 ctgggcaaca atttgctgta aaaattgttg atgtagccaa gttcacatca agtccagggt
      301 taagtacaga agatctaaag cgggaagcca gtatctgtca tatgctgaaa catccacaca
      361 ttgtagagtt attggagaca tatagctcag atggaatgct ttacatggtt ttcgaattta
      421 tggatggagc agatctgtgt tttgaaatcg taaagcgagc tgacgctggt tttgtgtaca
      481 gtgaagctgt agccagccat tatatgagac agatactgga agctctacgc tactgccatg
      541 ataataacat aattcacagg gatgtgaagc cccactgtgt tctccttgcc tcaaaagaaa
      601 actcggcacc tgttaaactt ggaggctttg gggtagctat tcaattaggg gagtctggac
      661 ttgtagctgg aggacgtgtt ggaacacctc attttatggc accagaagtg gtcaaaagag
      721 agccttacgg aaagcctgta gacgtctggg ggtgcggtgt gatccttttt atcctgctca
      781 gtggttgttt gcctttttac ggaaccaagg aaagattgtt tgaaggcatt attaa
//
