LOCUS       sequence-156115-       10572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10572)
  AUTHORS   Dulyaninova NG, House RP, Betapudi V, Bresnick AR
  TITLE     Myosin-IIA heavy-chain phosphorylation regulates the motility of 
            MDA-MB-231 carcinoma cells.
  JOURNAL   Mol Biol Cell. 2007 Aug;18(8):3144-55. Epub 2007 Jun 13.
  PUBMED    17567956
REFERENCE   2  (bases 1 to 10572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-08"; volume: "18(8)"; pages: "3144-55. Epub 2007
            Jun 13"
COMMENT     Sequence Label: pCMV-mCherry-MHC-IIA
FEATURES             Location/Qualifiers
     source          1..10572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      614..623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             620..1327
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSLNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1033..1052
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7367..7488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7404..7423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7458..7477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7495..7950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7632..7653)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7844..7863
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7977..8081
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8083..8440
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8083..8274
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8106..8126)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8291..8426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8475..9269
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8475..9269
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8529..8548)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9139..9158
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9457..9476)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9501..9548
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9877..10465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10366..10385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gtagccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      661 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      781 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      841 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      961 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     1021 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     1081 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     1141 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     1201 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tccctcaacg aggactacac
     1261 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     1321 gtacaagaag cttatggcac agcaagctgc cgataagtat ctctatgtgg ataaaaactt
     1381 catcaacaat ccgctggccc aggccgactg ggctgccaag aagctggtat gggtgccttc
     1441 cgacaagagt ggctttgagc cagccagcct caaggaggag gtgggcgaag aggccatcgt
     1501 ggagctggtg gagaatggga agaaggtgaa ggtgaacaag gatgacatcc agaagatgaa
     1561 cccgcccaag ttctccaagg tggaggacat ggcagagctc acgtgcctca acgaagcctc
     1621 ggtgctgcac aacctcaagg agcgttacta ctcagggctc atctacacct attcaggcct
     1681 gttctgtgtg gtcatcaatc cttacaagaa cctgcccatc tactctgaag agattgtgga
     1741 aatgtacaag ggcaagaaga ggcacgagat gccccctcac atctatgcca tcacagacac
     1801 cgcctacagg agtatgatgc aagaccgaga agatcaatcc atcttgtgca ctggtgaatc
     1861 tggagctggc aagacggaga acaccaagaa ggtcatccag tatctggcgt acgtggcgtc
     1921 ctcgcacaag agcaagaagg accagggcga gctggagcgg cagctgctgc aggccaaccc
     1981 catcctggag gccttcggga acgccaagac cgtgaagaat gacaactcct cccgcttcgg
     2041 caaattcatt cgcatcaact ttgatgtcaa tggctacatt gttggagcca acattgagac
     2101 ttatcttttg gagaaatctc gtgctatccg ccaagccaag gaagaacgga ccttccacat
     2161 cttctattat ctcctgtctg gggctggaga gcacctgaag accgatctcc tgttggagcc
     2221 gtacaacaaa taccgcttcc tgtccaatgg acacgtcacc atccccgggc agcaggacaa
     2281 ggacatgttc caggagacca tggaggccat gaggattatg ggcatcccag aagaggagca
     2341 aatgggcctg ctgcgggtca tctcaggggt tcttcagctc ggcaacatcg tcttcaagaa
     2401 ggagcggaac actgaccagg cgtccatgcc cgacaacaca gctgcccaaa aggtgtccca
     2461 tctcttgggt atcaatgtga ccgatttcac cagaggaatc ctcaccccgc gcatcaaggt
     2521 gggacgggat tacgtccaga aggcgcagac taaagagcag gctgactttg ccatcgaggc
     2581 cttggccaag gcgacctatg agcggatgtt ccgctggctg gtgctgcgca tcaacaaggc
     2641 tctggacaag accaagaggc agggcgcctc cttcatcggg atcctggaca ttgccggctt
     2701 cgagatcttt gatctgaact cgtttgagca gctgtgcatc aattacacca atgagaagct
     2761 gcagcagctc ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgcgaggg
     2821 catcgagtgg aacttcatcg actttggcct cgacctgcag ccctgcatcg acctcattga
     2881 gaagccagca ggccccccgg gcattctggc cctgctggac gaggagtgct ggttccccaa
     2941 agccaccgac aagagcttcg tggagaaggt gatgcaggag cagggcaccc accccaagtt
     3001 ccagaagccc aagcagctga aggacaaagc tgatttctgc attatccact atgccggcaa
     3061 ggtggattac aaagctgacg agtggctgat gaagaacatg gatcccctga atgacaacat
     3121 cgccacactg ctccaccagt cctctgacaa gtttgtctcg gagctgtgga aggatgtgga
     3181 ccgcatcatc ggcctggacc aggtggccgg catgtcggag accgcactgc ccggggcctt
     3241 caagacgcgg aagggcatgt tccgcactgt ggggcagctt tacaaggagc agctggccaa
     3301 gctgatggct acgctgagga acacgaaccc caactttgtc cgctgcatca tccccaacca
     3361 cgagaagaag gccggcaagc tggacccgca tctcgtgctg gaccagctgc gctgcaacgg
     3421 tgttctcgag ggcatccgta tctgccgcca gggcttcccc aacagggtgg tcttccagga
     3481 gtttcggcag agatatgaga tcctgactcc aaactccatt cccaagggtt tcatggacgg
     3541 gaagcaggcg tgcgtgctca tgataaaagc cctggagctc gacagcaatc tgtaccgcat
     3601 tggccagagc aaagtcttct tccgtgccgg tgtgctggcc cacctggagg aggagcgaga
     3661 cctgaagatc accgacgtca tcatagggtt ccaggcctgc tgcaggggct acctggccag
     3721 gaaagcattt gccaagcggc agcagcagct taccgccatg aaggtcctcc agcggaactg
     3781 cgctgcctac ctgaagctgc ggaactggca gtggtggcgg ctcttcacca aggtcaagcc
     3841 gctgctgcag gtgagccggc aggaggagga gatgatggcc aaggaggagg agctggtgaa
     3901 ggtcagagag aagcagctgg ctgcggagaa caggctcacg gagatggaga cgctgcagtc
     3961 tcagctcatg gcagagaaat tgcagctgca ggagcagctc caggcagaaa ccgagctgtg
     4021 tgccgaggct gaggagctcc gggcccgcct gaccgccaag aagcaggaat tagaagagat
     4081 ctgccatgac ctagaggcca gggtggagga ggaggaggag cgctgccagc acctgcaggc
     4141 ggagaagaag aagatgcagc agaacatcca ggagcttgag gagcagctgg aggaggagga
     4201 gagcgcccgg cagaagctgc agctggagaa ggtgaccacc gaggcgaagc tgaaaaagct
     4261 ggaggaggag cagatcatcc tggaggacca gaactgcaag ctggccaagg aaaagaaact
     4321 gctggaagac agaatagctg agttcaccac caacctcaca gaagaggagg agaaatctaa
     4381 gagcctcgcc aagctcaaga acaagcatga ggcaatgatc actgacttgg aagagcgcct
     4441 ccgcagggag gagaagcagc gacaggagct ggagaagacc cgccggaagc tggagggaga
     4501 ctccacagac ctcagcgacc agatcgccga gctccaggcc cagatcgcgg agctcaagat
     4561 gcagctggcc aagaaagagg aggagctcca ggccgccctg gccagagtgg aagaggaagc
     4621 tgcccagaag aacatggccc tcaagaagat ccgggagctg gaatctcaga tctctgaact
     4681 ccaggaagac ctggagtctg agcgtgcttc caggaataaa gctgagaagc agaaacggga
     4741 ccttggggaa gagctagagg cgctgaaaac agagttggag gacacgctgg attccacagc
     4801 tgcccagcag gagctcaggt caaaacgtga gcaggaggtg aacatcctga agaagaccct
     4861 ggaggaggag gccaagaccc acgaggccca gatccaggag atgaggcaga agcactcaca
     4921 ggccgtggag gagctggcgg agcagctgga gcagacgaag cgggtgaaag caaacctcga
     4981 gaaggcaaag cagactctgg agaacgagcg gggggagctg gccaacgagg tgaaggtgct
     5041 gctgcagggc aaaggggact cggagcacaa gcgcaagaaa gtggaggcgc agctgcagga
     5101 gctgcaggtc aagttcaacg agggagagcg cgtgcgcaca gagctggccg acaaggtcac
     5161 caagctgcag gtggagctgg acaacgtgac cgggcttctc agccagtccg acagcaagtc
     5221 cagcaagctc accaaggact tctccgcgct ggagtcccag ctgcaggaca ctcaggagct
     5281 gctgcaggag gagaaccggc agaagctgag cctgagcacc aagctcaagc aggtggagga
     5341 cgagaagaat tccttccggg agcagctgga ggaggaggag gaggccaagc acaacctgga
     5401 gaagcagatc gccaccctcc atgcccaggt ggccgacatg aaaaagaaga tggaggacag
     5461 tgtggggtgc ctggaaactg ctgaggaggt gaagaggaag ctccagaagg acctggaggg
     5521 cctgagccag cggcacgagg agaaggtggc cgcctacgac aagctggaga agaccaagac
     5581 gcggctgcag caggagctgg acgacctgct ggtggacctg gaccaccagc gccagagcgc
     5641 gtgcaacctg gagaagaagc agaagaagtt tgaccagctc ctggcggagg agaagaccat
     5701 ctctgccaag tatgcagagg agcgcgaccg ggctgaggcg gaggcccgag agaaggagac
     5761 caaggctctg tcgctggccc gggccctgga ggaagccatg gagcagaagg cggagctgga
     5821 gcggctcaac aagcagttcc gcacggagat ggaggacctt atgagctcca aggatgatgt
     5881 gggcaagagt gtccacgagc tggagaagtc caagcgggcc ctagagcagc aggtggagga
     5941 gatgaagacg cagctggaag agctggagga cgagctgcag gccaccgaag atgccaagct
     6001 gcggttggag gtcaacctgc aggccatgaa ggcccagttc gagcgggacc tgcagggccg
     6061 ggacgagcag agcgaggaga agaagaagca gctggtcaga caggtgcggg agatggaggc
     6121 agagctggag gacgagagga agcagcgctc gatggcagtg gccgcccgga agaagctgga
     6181 gatggacctg aaggacctgg aggcgcacat cgactcggcc aacaagaacc gggacgaagc
     6241 catcaaacag ctgcggaagc tgcaggccca gatgaaggac tgcatgcgcg agctggatga
     6301 cacccgcgcc tctcgtgagg agatcctggc ccaggccaaa gagaacgaga agaagctgaa
     6361 gagcatggag gccgagatga tccagttgca ggaggaactg gcagccgcgg agcgtgccaa
     6421 gcgccaggcc cagcaggagc gggatgagct ggctgacgag atcgccaaca gcagcggcaa
     6481 aggagccctg gcgttagagg agaagcggcg tctggaggcc cgcatcgccc agctggagga
     6541 ggagctggag gaggagcagg gcaacacgga gctgatcaac gaccggctga agaaggccaa
     6601 cctgcagatc gaccagatca acaccgacct gaacctggag cgcagccacg cccagaagaa
     6661 cgagaatgct cggcagcagc tggaacgcca gaacaaggag cttaaggtca agctgcagga
     6721 gatggagggc actgtcaagt ccaagtacaa ggcctccatc accgccctcg aggccaagat
     6781 tgcacagctg gaggagcagc tggacaacga gaccaaggag cgccaggcag cctgcaaaca
     6841 ggtgcgtcgg accgagaaga agctgaagga tgtgctgctg caggtggatg acgagcggag
     6901 gaacgccgag cagtacaagg accaggccga caaggcatct acccgcctga agcagctcaa
     6961 gcggcagctg gaggaggccg aagaggaggc ccagcgggcc aacgcctccc gccggaaact
     7021 gcagcgcgag ctggaggacg ccactgagac ggccgatgcc atgaaccgcg aagtcagctc
     7081 cctaaagaac aagctcaggc gcggggacct gccgtttgtc gtgccccgcc gaatggcccg
     7141 gaaaggcgcc ggggatggct ccgacgaaga ggtagatggc aaagcggatg gggctgaggc
     7201 caaacctgcc gaataagtcg acggtaccgc gggcccggga tccaccggat ctagataact
     7261 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     7321 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     7381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     7501 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7561 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7621 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7681 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     7741 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     7801 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7861 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7921 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     7981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8041 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     8101 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8341 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     8461 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8521 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     8581 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     8641 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8701 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     8761 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8821 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8881 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8941 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     9001 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     9061 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     9121 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9181 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9241 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     9301 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     9361 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9421 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     9481 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     9541 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     9601 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     9661 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     9721 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9781 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10021 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10501 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10561 ccgtattacc gc
//
