LOCUS       sequence-156116-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses under the CAG promoter RandE3, a synthetic E3 ligase with 
            random residues in its binding domain; control construct for GFE3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts 
            RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, 
            Arnold DB
  TITLE     An E3-ligase-based method for ablating inhibitory synapses.
  JOURNAL   Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.
  PUBMED    27271196
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894."
COMMENT     Sequence Label: pCAG_EGFP-RandE3
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             334..414
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gacgagctgt acggtggagg ttctggtgga ggttctggag gtggatcagg aggaggttcg
       61 caattgctcg aagtcgtcgt tgcgactccg accagcctcc taatcagctg gcgggcgacg
      121 caggagcacg cgcgctacta ccgcatcacc tacggtgaaa ctggtggcaa tagccctgtc
      181 caggaattca ccgtgcctgg cagcaagtcc actgctacca tcagcggcct gaaacctggt
      241 gtcgactata ccatcacggt gtacgccgtc acgcgcgagc cccaggccag ctccgaccag
      301 tccccgatct ccatcaacta ccgcaccaga tcttacccat acgatgttcc agattacgct
      361 tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta cgctgctagc
      421 ctaaggcgcc tacaagagga gaagctttgc aaaatctgta tggatagaaa tattgctata
      481 gtttttgttc cttgtggaca tctggtcact tgtaaacagt gtgcggaagc agttgacaaa
      541 tgtcccatgt gctgcacagt cattacgttc aagcaaaaaa tttttatgtc ttaataatgt
      601 acaagtaaag cggccccgga ctcgaggccg caggtaagta tcaaggttac aagacaggtt
      661 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
      721 gcacctattg gtcttactga catccacttt gcctttctct c
//
