LOCUS       sequence-156123-        5683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Creasy CL, Ambrose DM, Chernoff J
  TITLE     The Ste20-like protein kinase, Mst1, dimerizes and contains an 
            inhibitory domain.
  JOURNAL   J Biol Chem. 1996 Aug 30. 271(35):21049-53.
  PUBMED    8702870
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1996 Aug 30. 271(35):21049-53."
COMMENT     Sequence Label: pJ3M-Mst2
FEATURES             Location/Qualifiers
     source          1..5683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     231..253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(396..416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        401..730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      581..716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             750..779
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     RBS             1448..1456
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(2811..2827)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2812..2828)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          2922..2987
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3117..3137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3562..3696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3599..3618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3653..3672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3727..3831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3832..3900,3901..4692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4050..4069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4862..5450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5351..5370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5604..5621
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctag aaactgctga
      301 gggctggacc gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt
      361 accacagata tcctgtttgg cccatattca gctgtggaat gtgtgtcagt tagggtgtgg
      421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      721 cttttgcaaa agcttcgatc gtcgacatgg aacagaaact catctctgaa gaggatctgt
      781 ctagaggatc cgccatggag cagccgccgg cgcctaagag taaactaaaa aagctgagtg
      841 aagacagttt gactaagcag cctgaagaag tttttgatgt attagagaag cttggagaag
      901 ggtcttatgg aagtgtattt aaagcaatac acaaggaatc cggtcaagtt gtcgcaatta
      961 aacaagtacc tgttgaatca gatcttcagg aaataatcaa agaaatttcc ataatgcagc
     1021 aatgtgacag cccatatgtt gtaaagtact atggcagtta ttttaagaat acagacctct
     1081 ggattgttat ggagtactgt ggcgctggct ctgtctcaga cataattaga ttacgaaaca
     1141 agacattaat agaagatgaa attgcaacca ttcttaaatc tacattgaaa ggactagaat
     1201 atttgcactt tatgagaaaa atacacagag atataaaagc tggaaatatt ctcctcaata
     1261 cagaaggaca tgcaaaattg gcagattttg gagtggctgg tcagttaaca gatacaatgg
     1321 caaaacgcaa tactgtaata ggaactccat tttggatggc tcctgaggtg attcaagaaa
     1381 taggctataa ctgtgtggcc gacatctggt cccttggcat tacttctata gaaatggctg
     1441 aaggaaaacc tccttatgct gatatacatc caatgagggc tatttttatg attcccacaa
     1501 atccaccacc aacattcaga aagccagaac tttggtccga tgatttcacc gattttgtta
     1561 aaaagtgttt ggtgaagaat cctgagcaga gagctactgc aacacaactt ttacagcatc
     1621 cttttatcaa gaatgccaaa cctgtatcaa tattaagaga cctgatcaca gaagctatgg
     1681 agatcaaagc taaaagacat gacgaacagc aacgagaatt ggaagaggaa gaagaaaatt
     1741 cggatgaaga tgagctggat tcccacacca tggtgaagac tagtgtggag agtgtgggca
     1801 ccatgcgggc cacaagcacg atgagtgaag gggcccagac catgattgaa cataatagca
     1861 cgatgttgga atccgacttg gggaccatgg tgataaacag tgaggatgag gaagaagaag
     1921 atggaactat gaaaagaaat gcaacctcac cacaagtaca aagaccatct ttcatggact
     1981 actttgataa gcaagacttc aagaataaga gtcacgaaaa ctgtaatcag aacatgcatg
     2041 aacccttccc tatgtccaaa aacgtttttc ctgataactg gaaagttcct caagatggag
     2101 actttgactt tttgaaaaat ctaagtttag aagaactaca gatgcggtta aaagcactgg
     2161 accccatgat ggaacgggag atagaagaac ttcgtcagag atacactgcg aaaagacagc
     2221 ccattctgga tgcgatggat gcaaagaaaa gaaggcagca aaacttttga gtctaatttc
     2281 ctctctgttt ttaactattc tggagaccaa gaaaccacta ggaattgaag gaatatttgg
     2341 atatttttaa tcctaagatt ttgccctaca attaggcaga ggtcaaaaag tgacaatggt
     2401 acatgcccag gtaaattccc aaaaggcaga attgacagtt gtatctgctg tgcattcact
     2461 ctaagatgag gagaacaaaa gaagtgtatt ctcttgttct gtcagctgca taccagtaat
     2521 aaaactgtta tgaaatggat tttcaaggtc tctaaacctt gaaaatccaa agctattgtt
     2581 gcattgtaca gcactgaagg gctttatgtt acaatattct ttattcctat ctagtatact
     2641 aggctattta ttgtatcccc ttaggtaaac ttatttattt atgctatttt gctttgtttc
     2701 attttttaag gacaagatca ggatagcttt ggtgaaggta gggtcatatt aatatgatga
     2761 taatgtgcaa ccaatttata ctttctgcag gaattcgata tcaagcttat cgataccgtc
     2821 gacctcgagg gggggcccgg taccagatct ttgtgaagga accttacttc tgtggtgtga
     2881 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     2941 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     3001 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     3061 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     3121 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     3181 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     3241 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     3301 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     3361 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     3421 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     3481 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3541 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3601 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3661 tccaaactca tcaatgtatc ttatcatgtc tggatctctg caggtggcac ttttcgggga
     3721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4021 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4081 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4141 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4201 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4261 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4321 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4381 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4441 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4501 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4561 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4621 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4681 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4741 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4801 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4861 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4921 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4981 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5041 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5101 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5161 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5221 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5281 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5341 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5401 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5461 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5521 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5581 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     5641 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccg
//
