LOCUS       sequence-156132-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian GFP reporter plasmid, target site for Vika recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Karimova M, Abi-Ghanem J, Berger N, Surendranath V, Pisabarro MT, 
            Buchholz F
  TITLE     Vika/vox, a novel efficient and specific Cre/loxP-like site-specific
            recombination system.
  JOURNAL   Nucleic Acids Res. 2013 Jan;41(2):e37. doi: 10.1093/nar/gks1037. 
            Epub 2012 Nov 9.
  PUBMED    23143104
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-01"; volume: "41(2):e37. doi"; pages: " 
            10.1093/nar/gks1037. Epub 2012 Nov 9"
COMMENT     Sequence Label: pRK5-vox-EGFP
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(48..77)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(84..797,798..800,801..803))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(87..797,798..800,801..803))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(129..150)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     478..497
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     737..758
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    892..939
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     964..983
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag cttacagcag gtcctcctcg
       61 ctgatcagct tctgctcgtc gacttacttg tacagctcgt ccatgccgag agtgatcccg
      121 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      181 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      241 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      301 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      361 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      421 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      481 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      541 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      601 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      661 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      721 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      781 agctcctcgc ccttgctcac catggatccc tcgagaatac gtctgagaat gggcgttctc
      841 agacctattt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
      901 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
      961 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca
     1021 tgagatcccc gcgctggagg atcatccagc cggcgtcccg ga
//
