LOCUS       sequence-156161-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral lncRNA Overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Emmrich S, Streltsov A, Schmidt F, Thangapandi VR, Reinhardt D, 
            Klusmann JH
  TITLE     LincRNAs MONC and MIR100HG act as oncogenes in acute 
            megakaryoblastic leukemia.
  JOURNAL   Mol Cancer. 2014 Jul 15;13:171. doi: 10.1186/1476-4598-13-171.
  PUBMED    25027842
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Cancer."; date: "2014-07-15"; volume: "13:171. doi"; pages: " 
            10.1186/1476-4598-13-171"
COMMENT     Sequence Label: LeGO-lnc
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    120..153
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(184..579)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(580..831)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(622..643)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
       61 gattatcgat aagcttgata tcgaattgta cctagtggaa ccggaaccct taaacatgta
      121 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgacgaat tcttacttgt
      181 acattagccc tcccacacat aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg
      241 ctgtccatca ctgtccttca ctatggcttt gatcccagga tgcagatcga gaagcacctg
      301 tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca tttccgatcg cgacgataca
      361 agtcaggttg ccagctgccg cagcagcagc agtgcccagc accacgagtt ctgcacaagg
      421 tcccccagta aaatgatata cattgacacc agtgaagatg cggccgtcgc tagagagagc
      481 tgcgctggcg acgctgtagt cttcagagat ggggatgctg ttgattgtag ccgttgctct
      541 ttcaatgagg gtggattctt cttgagacaa aggcttggcc ttgtacagct cgtccatgcc
      601 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      661 ggggtctttg ctcagcttgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
      721 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      781 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
      841 atagacgttg tcgctgatgg cgttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      901 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      961 ctcggcgcg
//
