LOCUS       sequence-156165-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE79
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(32..134)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgtgccg atcaacgtct caggtctctg caagaattca agcttggaga ttacggatat
      181 gaatataggc atatccgtat ccgaattatc cgtttgacag ctagcaacga ttgtacaatt
      241 gcttctttaa aaaaggaaga aagaaagaaa gaaaagaatc aacatcagcg ttaacaaacg
      301 gccccgttac ggcccaaacg gtcatataga gtaacggcgt taagcgttga aagactccta
      361 tcgaaatacg taaccgcaaa cgtgtcatag tcagatcccc tcttccttca ccgcctcaaa
      421 cacaaaaata atcttctaca gcctatatat acaacccccc cttctatctc tcctttctca
      481 caattcatca tctttctttc tctaccccca attttaagaa atcctctctt ctcctcttca
      541 ttttcaaggt aaatctctct ctctctctct ctctctgtta ttccttgttt taattaggta
      601 tgtattattg ctagtttgtt aatctgctta tcttatgtat gccttatgtg aatatcttta
      661 tcttgttcat ctcatccgtt tagaagctat aaatttgttg atttgactgt gtatctacac
      721 gtggttatgt ttatatctaa tcagatatga atttcttcat attgttgcgt ttgtgtgtac
      781 caatccgaaa tcgttgattt tt
//
