LOCUS       sequence-156166-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE79
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      152..859
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
       61 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac
      121 cccaagaaga agaggaaggt gtgagcttgt cctgctttaa tgagatatgc gagaagccta
      181 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg
      241 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat
      301 cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg
      361 tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat
      421 agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc
      481 ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg
      541 ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga
      601 actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc
      661 gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga
      721 cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc
      781 aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca
      841 acatgtcgag gctcagcagg accgctacta atcatgagcg gagaattaag ggagtcacgt
      901 tatgaccccc gccgatga
//
