LOCUS       sequence-156168-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, activator]; p35S:Cas9 
            D10A/N863A-Hax3CT, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG250
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        267..612
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     555..575
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             709..729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct caggtctctg caagaattca
      181 agcttggagg tcaacatggt ggagcacgac actctggtct actccaaaaa tgtcaaagat
      241 acagtctcag aagatcaaag ggctattgag acttttcaac aaaggataat ttcgggaaac
      301 ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa
      361 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatctctct
      421 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagag
      481 gttccaacca cgtctacaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
      541 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
      601 ttggagagga cacgctcgag tataagagct catttttaca acaattacca acaacaacaa
      661 acaacaaaca acattacaat tacatttaca attatcgata caatggctcc taagaagaag
      721 cggaaggttg gtggcatgga caagaagtac tccattgggc tcgctatcgg cacaaacagc
      781 gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg
      841 ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc
      901 ggggagacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgc
//
