LOCUS       sequence-156171-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of STK10. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK10_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca agaaagtccc gcgaatatga gcacgtccgc cgcgacctgg
      181 accccaacga ggtgtgggag atcgtgggcg agctgggcga cggcgccttc ggcaaggttt
      241 acaaggccaa gaataaggag acgggtgctt tggctgcggc caaagtcatt gaaaccaaga
      301 gtgaggagga gctggaggac tacatcgtgg agattgagat cctggccacc tgcgaccacc
      361 cctacattgt gaagctcctg ggagcctact atcacgacgg gaagctgtgg atcatgattg
      421 agttctgtcc agggggagcc gtggacgcca tcatgctgga gctggacaga ggcctcacgg
      481 agccccagat acaggtggtt tgccgccaga tgctagaagc cctcaacttc ctgcacagca
      541 agaggatcat ccaccgagat ctgaaagctg gcaacgtgct gatgaccctc gagggagaca
      601 tcaggctggc tgactttggt gtgtctgcca agaatctgaa gactctacag aaacgagatt
      661 ccttcatcgg cacgccttac tggatggccc ccgaggtggt catgtgtgag accatgaaag
      721 acacgcccta cgactacaaa gccgacatct ggtccctggg catcacgctg attgagatgg
      781 cccagatcga gccgccacac cacgagctca accccatgcg ggtc
//
