LOCUS       sequence-156175-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of VGFR1. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: VGFR1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca ccagatgaag ttcctttgga tgagcagtgt gagcggctcc
      181 cttatgatgc cagcaagtgg gagtttgccc gggagagact taaactgggc aaatcacttg
      241 gaagaggggc ttttggaaaa gtggttcaag catcagcatt tggcattaag aaatcaccta
      301 cgtgccggac tgtggctgtg aaaatgctga aagagggggc cacggccagc gagtacaaag
      361 ctctgatgac tgagctaaaa atcttgaccc acattggcca ccatctgaac gtggttaacc
      421 tgctgggagc ctgcaccaag caaggagggc ctctgatggt gattgttgaa tactgcaaat
      481 atggaaatct ctccaactac ctcaagagca aacgtgactt attttttctc aacaaggatg
      541 cagcactaca catggagcct aagaaagaaa aaatggagcc aggcctggaa caaggcaaga
      601 aaccaagact agatagcgtc accagcagcg aaagctttgc gagctccggc tttcaggaag
      661 ataaaagtct gagtgatgtt gaggaagagg aggattctga cggtttctac aaggagccca
      721 tcactatgga agatctgatt tcttacagtt ttcaagtggc cagaggcatg gagttcctgt
      781 cttccagaaa gtgcattcat cgggacctgg cagcgagaaa cattctttta tctgagaaca
//
