LOCUS       sequence-156177-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1E. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1E_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggagctac gtgtggggaa caagtaccgc ctgggacgga
      181 agatcgggag cgggtccttc ggagatatct acctgggtgc caacatcgcc tctggtgagg
      241 aagtcgccat caagctggag tgtgtgaaga caaagcaccc ccagctgcac atcgagagca
      301 agttctacaa gatgatgcag ggtggcgtgg ggatcccgtc catcaagtgg tgcggagctg
      361 agggcgacta caacgtgatg gtcatggagc tgctggggcc tagcctcgag gacctgttca
      421 acttctgttc ccgcaaattc agcctcaaga cggtgctgct cttggccgac cagatgatca
      481 gccgcatcga gtatatccac tccaagaact tcatccaccg ggacgtcaag cccgacaact
      541 tcctcatggg gctggggaag aagggcaacc tggtctacat catcgacttc ggcctggcca
      601 agaagtaccg ggacgcccgc acccaccagc acattcccta ccgggaaaac aagaacctga
      661 ccggcacggc ccgctacgct tccatcaaca cgcacctggg cattgagcaa agccgtcgag
      721 atgacctgga gagcctgggc tacgtgctca tgtacttcaa cctgggctcc ctgccctggc
      781 aggggctcaa agcagccacc aagcgccaga agtatga
//
