LOCUS       sequence-156183-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1D. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1D_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggagctga gagtcgggaa caggtaccgg ctgggccgga
      181 agatcggcag cggctccttc ggagacatct atctcggtac ggacattgct gcaggagaag
      241 aggttgccat caagcttgaa tgtgtcaaaa ccaaacaccc tcagctccac attgagagca
      301 aaatctacaa gatgatgcag ggaggagtgg gcatccccac catcagatgg tgcggggcag
      361 agggggacta caacgtcatg gtgatggagc tgctggggcc aagcctggag gacctcttca
      421 acttctgctc caggaaattc agcctcaaaa ccgtcctgct gcttgctgac caaatgatca
      481 gtcgcatcga atacattcat tcaaagaact tcatccaccg ggatgtgaag ccagacaact
      541 tcctcatggg cctggggaag aagggcaacc tggtgtacat catcgacttc gggctggcca
      601 agaagtaccg ggatgcacgc acccaccagc acatccccta tcgtgagaac aagaacctca
      661 cggggacggc gcggtacgcc tccatcaaca cgcaccttgg aattgaacaa tcccgaagag
      721 atgacttgga gtctctgggc tacgtgctaa tgtacttcaa cctgggctct ctcccctggc
      781 aggg
//
