LOCUS       sequence-156187-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MERTK. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MERTK_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca agtgaggaac tacaaaataa actagaagat gttgtgattg
      181 acaggaatct tctaattctt ggaaaaattc tgggtgaagg agagtttggg tctgtaatgg
      241 aaggaaatct taagcaggaa gatgggacct ctctgaaagt ggcagtgaag accatgaagt
      301 tggacaactc ttcgcagcgg gagatcgagg agtttctcag tgaggcagcg tgcatgaaag
      361 acttcagcca cccaaatgtc attcgacttc taggtgtgtg tatagaaatg agctctcaag
      421 gcatcccaaa gcccatggta attttaccct tcatgaaata cggggacctg catacttact
      481 tactttattc ccgattggag acaggaccaa agcatattcc tctgcagaca ctattgaagt
      541 tcatggtgga tattgccctg ggaatggagt atctgagcaa caggaatttt cttcatcgag
      601 atttagctgc tcgaaactgc atgttgcgag atgacatgac tgtctgtgtt gcggacttcg
      661 gcctctctaa gaagatttac agtggcgatt attaccgcca aggccgcatt gctaagatgc
      721 ctgttaaatg gatcgccata gaaagtcttg cagaccgagt ctacacaagt aaaagtgatg
      781 tgtgggcatt tggcgtgacc atgtgggaaa tagctacgcg g
//
