LOCUS       sequence-156190-       10697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of NHA-tagged human-Nematostella GW182 
            chimera in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1537
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     681..701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     727..751
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          891..1023
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1006..1025
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1068..1086
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1090..1099
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1096..1161
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1189..1215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6402..6523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6413..6432)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6467..6486
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6709..7164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6796..6815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7006..7027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7281..7638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7281..7472
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7304..7324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7489..7624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7689..8483
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7743..7762)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8353..8372
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8547..8595
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8636..8655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8755..8777
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8815..8833)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8901..9005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9006..9074,9075..9866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9224..9243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10037..10625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10526..10545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
       61 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      121 caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 gcgatcgccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      721 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
      781 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
      841 tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
      901 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
      961 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1021 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac
     1081 tataggctag ccaccatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa
     1141 gctcaatgga aagctgcaaa cggtggatct cctccaagct tgatggctta cccatacgat
     1201 gttccagatt acgctcctgc aggcatggat gctgattctg cctccagttc tgaatcagag
     1261 agaaacatca ctatcatggc ttcagggaac acaggtggtg aaaaagatgg ccttcggaat
     1321 agcactggac ttggttccca aaacaagttt gtagttggta gcagcagcaa taatgtgggc
     1381 catggaagta gtactgggcc atggggtttt tcccatggag ccataataag cacatgtcag
     1441 gtctctgtgg atgctcctga aagcaaatct gaaagtagca acaatagaat gaatgcttgg
     1501 ggcactgtaa gttcttcatc aaatggaggg ttaaatccaa gcactttgaa ttcagctagc
     1561 aaccatggtg cctggccagt attagagaac aatggacttg ccctaaaagg gcctgtaggg
     1621 agtggtagtt ctggcattaa tattcagtgc agtactatag gccagatgcc taacaatcag
     1681 agtattaact ctaaagtgag tggtggttct acccatggta cctggggaag ccttcaggaa
     1741 acttgtgaat ctgaagtaag tggtacacag aaggtttcat tcagtggtca acctcaaaat
     1801 attaccactg aaatgactgg accaaataac actactaact ttatgacctc tagtttacca
     1861 aactccggtt cagtgcagaa taatgagctg cctagtagta acacaggggc ctggcgtgtg
     1921 agcacaatga atcatcctca gatgcaggct ccatcaggta tgaatggcac ttccctttct
     1981 caccttagca atggagagtc aaaaagtgga ggctcttatg gtactacatg gggtgcctat
     2041 ggttctaatt actctggaga caaatgttca ggccctaatg gccaagctaa tggtgacact
     2101 gtgaatgcaa ctctaatgca gcctggcgta aatggtccta tgggcactaa ctttcaagtt
     2161 aacacaaaca aaggaggtgg tgtgtgggaa tctggtgcag caaactccca gagtacatca
     2221 tggggaagtg gaaatggcgc aaattctgga ggaagtcgaa gaggatgggg aacccctgca
     2281 caaaacactg gcactaattt acccagcgtt gagtggaaca aactgcctag caatcagcat
     2341 tccaatgata gtgcaaatgg caatggtaag acgtttacaa atggatggaa atctactgag
     2401 gaagaggatc agggttctgc cacatctcag acaaatgagc aaagcagtgt gtgggccaaa
     2461 acaggaggta cagtggagag cgatggtagt acagaaagca ctggacgcct tgaggaaaaa
     2521 ggaactgggg aaagtcagag tagagacaga agaaaaattg atcagcacac attactccaa
     2581 agcattgtaa acagaactga cttagatcca cgtgtcctgt ccaactctgg ttggggacag
     2641 actcctatta agcagaatac tgcctgggat acagaaacat cacctagagg ggaacgaaag
     2701 actgacaatg ggacagaggc ctggggaagc tctgcaacac agacttttaa ctcaggggca
     2761 tgtatagata agactagccc taatggtaat gatacctcat ctgtatcagg gtggggcgat
     2821 cccaaacctg ctctgaggtg gggagattcc aaaggctcaa actgccaggg ggggtgggaa
     2881 gatgattctg ctgctacagg aatggtcaag agcaatcagt gggggaattg caaagaggag
     2941 aaggctgcat ggaatgactc gcaaaagaat aaacagggat ggggtgatgg acaaaaatca
     3001 agccaagggt ggtctgtttc tgccagtgat aactggggag aaacttcaag gaataaccat
     3061 tggggtgagg ccaataagaa atccagctca ggaggtagtg acagtgacag gtccgtttcc
     3121 ggttggaacg aacttggtaa aactagttct ttcacttggg gaaacaacat aaatccaaat
     3181 aattcatcag gatgggatga atcttctaaa cctactcctt cccagggatg gggagaccct
     3241 ccaaagtcta atcagtctct aggttgggga gattcgtcaa agccagtcag ctctccagac
     3301 tggaacaagc aacaagacat tgttggatct tggggaatcc caccagctac aggcaaacct
     3361 cctggtacag gctggctggg gggacctata ccagccccag caaaagaaga agaacccaca
     3421 ggctgggagg aaccatcccc agaatctata cgtcgcaaaa tggagattga tgatggaact
     3481 tcagcttggg gagatccaag caaatacaac tacaaaaatg tgaacatgtg gaacaaaaac
     3541 gtcccaaatg gcaacagccg ttcagaccag caagcacagg tacatcagct gctaacgcct
     3601 gcaagtgcca tctcaaacaa agaggcaagc agtggctctg gctggggtga gccctggggg
     3661 gagccttcta ctccagccac aactgtggat aatggtactt cagcatgggg taagcccata
     3721 gacagtggtc ccagctgggg ggaacccatt gctgcggcat ccagcacatc cacgtggggc
     3781 tccagctctg ttggtccaca agcattaagc aaatctgggc caaaatctat gcaagatggc
     3841 tggtgtggtg atgatatgcc attgcctgga aatcgcccca ctggctggga agaggaagag
     3901 gatgtggaga ttggaatgtg gaatagtaat tcatctcaag agcttaactc atctttaaat
     3961 tggccaccat atacaaagaa aatgtcatcg aagggtctga gtggcaaaaa aaggagaagg
     4021 gaaaggggaa tgatgaaagg tggaaacaaa caagaagaag cgtggataaa tccatttgtt
     4081 aaacagtttt caaacatcag tttttcgaga gactcaccag aggaaaatgt acaaagcaat
     4141 aagatggacc tttctggagg aatgttacaa gacaaacgaa tggagataga taaacatagc
     4201 ctaaatattg gtgattacaa tcgaacggtc gggaaaggcc ctggttctcg gcctcagatt
     4261 tccaaagagt cttccatgga gcgcaatcct tattttgata aggatggcat tgtagcagat
     4321 gaatcccaaa acatgcagtt tatgtccagt caaagcatga agcttccccc ttcaaatagt
     4381 gcactaccta accaggccct tggctccata gcagggctgg gtatgcaaaa cttgaattct
     4441 gttagacaga atggcaatcc cagtatgttt ggtgttggaa acacagcagc acaaccccgg
     4501 ggcatgcagc agcctccagc acaacctctt agttcatctc aaccaaactt aagagcacag
     4561 gtgcctcctc cattactctc ccctcaggtt ccagtttcat tgctgaagta tgcaccaaac
     4621 aacggtggcc tgaatccact ctttggccct caacaggtag ccatgctgaa ccagctatcc
     4681 cagctaaacc agctttctca gatctcccag ttacagcgat tgttagcgca ggccccacag
     4741 tccaggctga cccagtggaa acaaccactc ctccctgatc ccgagacctc tcatggcgaa
     4801 acatggggca tgcccaagat tgagcagatg tctctgctgt caggacctgt agtgagctcc
     4861 agcctggaac agcagcatgg ggctcccaac ctttctccac aggcaaatag gattgccgat
     4921 cccgtgagct cacggtgggg cgttgacgcc tctcttaagc tcagcgccga ccctccagag
     4981 ttcaagcccg gagtcccctg gcgccctcgg ggggaggggg aagaaaagtc tggttcatct
     5041 ggaagtagta tggaatcatc ccctcagggc atgcctgcca gtggcaagac tcagataaca
     5101 gacaacccaa acaagcagcc gggctttcca ggcgggttta gcagcgctag ccagttcccg
     5161 tactctatag ccagcagtcc ctggcagaac actgaaccca cagatagcct gaacaagacc
     5221 caggcgaacc ccagtagtat cagaccacct ccagggctca atacatctac tagtagcggc
     5281 ctccccgata ccccctctgt cgaagagaga cctgcatgga tgaaaaatct catggatggg
     5341 gggacccagc agagctttgc ccagggacct aatcctggga actttcagct gggactgcgg
     5401 ttgggcttct ccgccagcgg caattcctgg agtacccagg acgggatgtc tgtttccgga
     5461 ctgactccta atagccaacc gtgggctgcc ccaggcggac ctagttcttc ctccacacca
     5521 tcctctgacc cctctgccga ggccgccagc tccaccgcca atgccgtaac aagtgctgaa
     5581 cagcttaaac ctcaggatga agggatctca agttggtcca ccaaccagcc aattccgtct
     5641 gcggaggaga aacaacagca gccacctgtg gcccaaggac ctaggatggg atccggtcgc
     5701 ggcggagcca acccaatgag tacttggctg gtgttgcgga atctgtcccc acgggcagat
     5761 ccgaccgcaa tgagggctgt gtgccagcag tacggcccac tcctcacctt tactctgaac
     5821 cttaggcacg gaaacagctt gatcagatac tctaataagg atcaagccgc ctcagcacgg
     5881 aacaacctca acgggatgat ggtgaaaggt atgcagctta tcgccgactt tgcgacggat
     5941 agcgatattg gggggttttt tgagcagaca cccgactggt ccaacaaccc aatgccaccc
     6001 aaccctagct tcggcaaccc ttggtccttc ggaacagttg atcctaatcg acctctcgga
     6061 tccactcaag gactggagaa aacgccgcaa ggccccccac ctcaaatgcc tgcaatggtt
     6121 ccgccgggca tgcaatgggg aagtagttct cagctccccg ctcaactctg gggcaactcc
     6181 catcctggtg ccgccacagg cttcccgtct caggtgccat ccatgtggtc tttcagcgga
     6241 ggagcccctc gcgatgtcga accccaatcc ggaacaaatg agaatgggtt ggtctctcca
     6301 tccatgacta cgtttctgcc tcccgggttg ctgaacggtg gtgagagtgt ctaaggcggc
     6361 cgcttccctt tagtgagggt taatgcttcg agcagacatg ataagataca ttgatgagtt
     6421 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6481 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6541 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     6601 ctacaaatgt ggtaaaatcc gataaggatc gatccgggct ggcgtaatag cgaagaggcc
     6661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     6721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6781 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6901 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7021 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7081 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7141 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     7201 tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga
     7261 ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     7321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7561 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7621 ggcctaggct tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta
     7681 gagccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7741 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7801 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7861 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7921 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7981 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8041 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8101 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8161 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     8221 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8281 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8341 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8401 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8461 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     8521 gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg
     8581 ttggtttttt gtgtgaatcg atagcgataa ggatccgcgt atggtgcact ctcagtacaa
     8641 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8701 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8761 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9241 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9541 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10681 acatggctcg acagatc
//
