LOCUS       sequence-156210-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of Gata3-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lazarus Gata 3 flag
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..88
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             85..93
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggtggacca tcctctagac tgccggatct aagtaagtag gatccattta aattcgaatt
       61 catggattac aaggatgacg acgataagga ggtgactgcg gaccagccgc gctgggtgag
      121 ccaccatcac cccgcggtcc tcaacggtca gcacccagac acgcaccacc cgggcctcgg
      181 ccattcgtac atggaagctc agtatccgct gacggaagag gtggacgtac tttttaacat
      241 cgatggtcaa ggcaaccacg tcccgtccta ctacggaaac tccgtcaggg ctacggtgca
      301 gaggtatcct ccgacccacc acgggagcca ggtatgccgc ccgcctctgc tgcacggatc
      361 tctgccctgg ctggatggcg gcaaagccct gagcagccac cacaccgcct cgccctggaa
      421 cctcagcccc ttctccaaga cgtccatcca ccacggctct ccggggcctc tgtccgttta
      481 ccctccggct tcatcctctt ctctggcggc cggccactcc agtcctcatc tcttcacctt
      541 cccgcccacc ccgccgaaag acgtctcccc agacccgtcg ctgtccaccc cgggatccgc
      601 cgggtcggcc aggcaagatg agaaagagtg cctcaagtat caggtgcagc tgccagatag
      661 catgaagctg gagacgtctc actctcgagg cagcatgacc accctgggtg gggcctcatc
      721 ctcagcccac caccccatta ccacctatcc gccctatgtg cccgagtaca gctctggact
      781 cttcccaccc agcagcctgc tgggaggatc ccctaccggg ttcggatgta agtcgaggcc
      841 caaggcacga tccagcacag aaggcaggga gtgtgtgaac tgcggggcaa cctctacccc
      901 actgtggcgg cgagatggta ccgggcacta cctttgcaat gcctgcggac tctaccataa
      961 aatgaatggg cagaaccggc cccttatcaa gcccaagcga ag
//
