LOCUS       sequence-156214-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 V33A and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 V33A Cas2
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..288
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(469..488)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
       61 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
      121 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
      181 ttaagattct tgtacgacgg tattagaatt caagctgatc aggcccctga agatttggac
      241 atggaggata acgatattat tgaggctcac cgcgaacaga ttggaggtcg gtttatcccc
      301 gctggcgcgg ggaactctct aaaagtatac atttgttctt aaagcatttt ttcccataca
      361 aacaacccac caaccttaat gtaacaggat ctggcagtag ccatattcaa cgggaaacgt
      421 cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      481 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
      541 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      601 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      661 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
      721 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
      781 gccggttgca ttcgattcct gtttgtaatt
//
