LOCUS       sequence-156221-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 V190I and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 V190I Cas2
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(460..479)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtcaatcaag aagctaagcc agaggtcaag ccagaagtca agcctgagac tcacatcaat
       61 ttaaaggtgt ccgatggatc ttcagagatc ttcttcaaga tcaaaaagac cactccttta
      121 agaaggctga tggaagcgtt cgctaaaaga cagggtaagg aaatggactc cttaagattc
      181 ttgtacgacg gtattagaat tcaagctgat caggcccctg aagatttgga catggaggat
      241 aacgatatta ttgaggctca ccgcgaacag attggaggtc ggtttatccc cgctggcgcg
      301 gggaactctc taaaagtata catttgttct taaagcattt tttcccatac aaacaaccca
      361 ccaaccttaa tgtaacagga tctggcagta gccatattca acgggaaacg tcttgctcta
      421 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      481 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      541 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      601 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      661 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
      721 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      781 attcgattcc tgtttgtaat tgtcctttta acagcgaccg cgtattt
//
