LOCUS       sequence-156224-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 P279L and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 P279L Cas2
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(461..480)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa
       61 tttaaaggtg tccgatggat cttcagagat cttcttcaag atcaaaaaga ccactccttt
      121 aagaaggctg atggaagcgt tcgctaaaag acagggtaag gaaatggact ccttaagatt
      181 cttgtacgac ggtattagaa ttcaagctga tcaggcccct gaagatttgg acatggagga
      241 taacgatatt attgaggctc accgcgaaca gattggaggt cggtttatcc ccgctggcgc
      301 ggggaactct ctaaaagtat acatttgttc ttaaagcatt ttttcccata caaacaaccc
      361 accaacctta atgtaacagg atctggcagt agccatattc aacgggaaac gtcttgctct
      421 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
      481 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
      541 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
      601 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
      661 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
      721 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
      781 cattcgattc ctgtttgtaa ttgtcctttt aacagcgacc gcgtatttcg tctcgctcag
      841 gcgcaatcac gaatgaataa cggtt
//
