LOCUS       sequence-156227-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 P212L and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 P212L Cas2
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..291
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(472..491)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 caggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
       61 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
      121 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
      181 tccttaagat tcttgtacga cggtattaga attcaagctg atcaggcccc tgaagatttg
      241 gacatggagg ataacgatat tattgaggct caccgcgaac agattggagg tcggtttatc
      301 cccgctggcg cggggaactc tctaaaagta tacatttgtt cttaaagcat tttttcccat
      361 acaaacaacc caccaacctt aatgtaacag gatctggcag tagccatatt caacgggaaa
      421 cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
      481 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
      541 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
      601 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
      661 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc
      721 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
      781 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgac cgcgtattt
//
