LOCUS       sequence-156228-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HO endonuclease expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   McCusker J
  TITLE     McCusker Lab Unpublished Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRY003
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     450..468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(506..528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     628..647
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        671..887
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tatgactaaa tctcattcag aagaagtggc tagcggatcg ccaacaaata ctacctttta
       61 tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgcgtagaag
      121 accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta
      181 tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt
      241 tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac
      301 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
      361 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa
      421 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
      481 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      541 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      601 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      661 ataccacctt ttcaattcat catttttttt tttattcttt tttttgattt cggtttcctt
      721 gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac
      781 ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct
      841 taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta
      901 catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca
      961 tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac
//
