LOCUS       sequence-156232-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of Gata2-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lazarus Gata 2 flag
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..89
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             86..94
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggggtggacc atcctctaga ctgccggatc taagtaagta ggatccattt aaattcgaat
       61 tcatggatta caaggatgac gacgataagg aggtggcgcc tgagcagcct cgctggatgg
      121 cgcaccccgc cgtattgaat gcgcagcacc ccgactcgca ccatccgggc ctggcgcata
      181 actacatgga gccagcacag ctgctgcctc ccgacgaggt ggatgtcttc ttcaaccatc
      241 tcgactcgca gggcaaccct tactacgcca acccggccca cgcgcgcgcg cgcgtttcct
      301 acagcccggc gcatgcccgt ctcaccggag gccagatgtg ccgaccacac ttgttgcaca
      361 gcccaggctt gccgtggctg gacgggggca aagcagctct ctctgccgcc gctgcccatc
      421 accacagtcc ctggaccgtc agcccgttct ccaagacccc gctgcacccc tcagctgctg
      481 gagcacccgg agggcctctg tctgtttacc caggggctgc gggtgggagc gggggaggca
      541 gtgggagctc cgtggcctcc ctcaccccca ctgcagccca ctcgggctcc catctcttcg
      601 gcttcccacc cacgccaccc aaagaagtgt ctccagaccc cagcacaaca ggagctgctt
      661 ccccggcctc ttcttctgca gggggtagtg tagcccgggg tgaggacaag gatggcgtca
      721 agtaccaagt gtcactctcc gagagcatga agatggaagg cggcagtccc ctgcgcccgg
      781 gcctagctac catgggcacc cagcctgcaa cacaccaccc gatacccacc tatccctcct
      841 atgtgc
//
