LOCUS       sequence-156241-        9162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6344 pcDNA4-TO-HA-Brd4FL
FEATURES             Location/Qualifiers
     source          1..9162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             989..1015
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3248..3265
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5170..5187)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5176..5400
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5446..5874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5533..5552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5743..5764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5883..5903)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5888..6217
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6068..6203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6130..6149
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6265..6312
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6331..6705
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6835..6956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6872..6891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6926..6945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7005..7021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7005..7021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7018..7040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7029..7045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7053..7059,7060..7077,7078..7083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7098..7119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7236..7253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7407..7995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7487..7506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8166..8957,8958..9026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8789..8808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9027..9131)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctatggagta cccatacgac gtaccagatt acgcttggta
     1021 ccgagctcgg atccggatgt ctgcggagag cggccctggg acgagattga gaaatctgcc
     1081 agtaatgggg gatggactag aaacttccca aatgtctaca acacaggccc aggcccaacc
     1141 ccagccagcc aacgcagcca gcaccaaccc cccgccccca gagacctcca accctaacaa
     1201 gcccaagagg cagaccaacc aactgcaata cctgctcaga gtggtgctca agacactatg
     1261 gaaacaccag tttgcatggc ctttccagca gcctgtggat gccgtcaagc tgaacctccc
     1321 tgattactat aagatcatta aaacgcctat ggatatggga acaataaaga agcgcttgga
     1381 aaacaactat tactggaatg ctcaggaatg tatccaggac ttcaacacta tgtttacaaa
     1441 ttgttacatc tacaacaagc ctggagatga catagtctta atggcagaag ctctggaaaa
     1501 gctcttcttg caaaaaataa atgagctacc cacagaagaa accgagatca tgatagtcca
     1561 ggcaaaagga agaggacgtg ggaggaaaga aacagggaca gcaaaacctg gcgtttccac
     1621 ggtaccaaac acaactcaag catcgactcc tccgcagacc cagacccctc agccgaatcc
     1681 tcctcctgtg caggccacgc ctcacccctt ccctgccgtc accccggacc tcatcgtcca
     1741 gacccctgtc atgacagtgg tgcctcccca gccactgcag acgcccccgc cagtgccccc
     1801 ccagccacaa cccccacccg ctccagctcc ccagcccgta cagagccacc cacccatcat
     1861 cgcggccacc ccacagcctg tgaagacaaa gaagggagtg aagaggaaag cagacaccac
     1921 cacccccacc accattgacc ccattcacga gccaccctcg ctgcccccgg agcccaagac
     1981 caccaagctg ggccagcggc gggagagcag ccggcctgtg aaacctccaa agaaggacgt
     2041 gcccgactct cagcagcacc cagcaccaga gaagagcagc aaggtctcgg agcagctcaa
     2101 gtgctgcagc ggcatcctca aggagatgtt tgccaagaag cacgccgcct acgcctggcc
     2161 cttctacaag cctgtggacg tggaggcact gggcctacac gactactgtg acatcatcaa
     2221 gcaccccatg gacatgagca caatcaagtc taaactggag gcccgtgagt accgtgatgc
     2281 tcaggagttt ggtgctgacg tccgattgat gttctccaac tgctataagt acaaccctcc
     2341 tgaccatgag gtggtggcca tggcccgcaa gctccaggat gtgttcgaaa tgcgctttgc
     2401 caagatgccg gacgagcctg aggagccagt ggtggccgtg tcctccccgg cagtgccccc
     2461 tcccaccaag gttgtggccc cgccctcatc cagcgacagc agcagcgata gctcctcgga
     2521 cagtgacagt tcgactgatg actctgagga ggagcgagcc cagcggctgg ctgagctcca
     2581 ggagcagctc aaagccgtgc acgagcagct tgcagccctc tctcagcccc agcagaacaa
     2641 accaaagaaa aaggagaaag acaagaagga aaagaaaaaa gaaaagcaca aaaggaaaga
     2701 ggaagtggaa gagaataaaa aaagcaaagc caaggaacct cctcctaaaa agacgaagaa
     2761 aaataatagc agcaacagca atgtgagcaa gaaggagcca gcgcccatga agagcaagcc
     2821 ccctcccacg tatgagtcgg aggaagagga caagtgcaag cctatgtcct atgaggagaa
     2881 gcggcagctc agcttggaca tcaacaagct ccccggcgag aagctgggcc gcgtggtgca
     2941 catcatccag tcacgggagc cctccctgaa gaattccaac cccgacgaga ttgaaatcga
     3001 ctttgagacc ctgaagccgt ccacactgcg tgagctggag cgctatgtca cctcctgttt
     3061 gcggaagaaa aggaaacctc aagctgagaa agttgatgtg attgccggct cctccaagat
     3121 gaagggcttc tcgtcctcag agtcggagag ctccagtgag tccagctcct ctgacagcga
     3181 agactccgaa acagagatgg ctccgaagtc aaaaaagaag gggcaccccg ggagggagca
     3241 gaagaagcac catcatcacc accatcagca gatgcagcag gccccggctc ctgtgcccca
     3301 gcagccgccc ccccctcccc agcagccccc accgcctcca cctccgcagc agcaacagca
     3361 gccgccaccc ccgcctcccc caccctccat gccgcagcag gcagccccgg cgatgaagtc
     3421 ctcgccccca cccttcattg ccacccaggt gcccgtcctg gagccccagc tcccaggcag
     3481 cgtctttgac cccatcggcc acttcaccca gcccatcctg cacctgccgc agcctgagct
     3541 gccccctcac ctgccccagc cgcctgagca cagcactcca ccccatctca accagcacgc
     3601 agtggtctct cctccagctt tgcacaacgc actaccccag cagccatcac ggcccagcaa
     3661 ccgagccgct gccctgcctc ccaagcccgc ccggccccca gccgtgtcac cagccttgac
     3721 ccaaacaccc ctgctcccac agccccccat ggcccaaccc ccccaagtgc tgctggagga
     3781 tgaagagcca cctgccccac ccctcacctc catgcagatg cagctgtacc tgcagcagct
     3841 gcagaaggtg cagcccccta cgccgctact cccttccgtg aaggtgcagt cccagccccc
     3901 accccccctg ccgcccccac cccacccctc tgtgcagcag cagctgcagc agcagccgcc
     3961 accaccccca ccaccccagc cccagcctcc accccagcag cagcatcagc cccctccacg
     4021 gcccgtgcac ttgcagccca tgcagttttc cacccacatc caacagcccc cgccacccca
     4081 gggccagcag cccccccatc cgcccccagg ccagcagcca cccccgccgc agcctgccaa
     4141 gcctcagcaa gtcatccagc accaccattc accccggcac cacaagtcgg acccctactc
     4201 aaccggtcac ctccgcgaag ccccctcccc gcttatgata cattcccccc agatgtcaca
     4261 gttccagagc ctgacccacc agtctccacc ccagcaaaac gtccagccta agaaacagga
     4321 gctgcgtgct gcctccgtgg tccagcccca gcccctcgtg gtggtgaagg aggagaagat
     4381 ccactcaccc atcatccgca gcgagccctt cagcccctcg ctgcggccgg agccccccaa
     4441 gcacccggag agcatcaagg cccccgtcca cctgccccag cggccggaaa tgaagcctgt
     4501 ggatgtcggg aggcctgtga tccggccccc agagcagaac gcaccgccac caggggcccc
     4561 tgacaaggac aaacagaaac aggagccgaa gactccagtt gcgcccaaaa aggacctgaa
     4621 aatcaagaac atgggctcct gggccagcct agtgcagaag catccgacca ccccctcctc
     4681 cacagccaag tcatccagcg acagcttcga gcagttccgc cgcgccgctc gggagaaaga
     4741 ggagcgtgag aaggccctga aggctcaggc cgagcacgct gagaaggaga aggagcggct
     4801 gcggcaggag cgcatgagga gccgagagga cgaggatgcg ctggagcagg cccggcgggc
     4861 ccatgaggag gcacgtcggc gccaggagca gcagcagcag cagcgccagg agcaacagca
     4921 gcagcagcaa cagcaagcag ctgcggtggc tgccgccgcc accccacagg cccagagctc
     4981 ccagccccag tccatgctgg accagcagag ggagttggcc cggaagcggg agcaggagcg
     5041 aagacgccgg gaagccatgg cagctaccat tgacatgaat ttccagagtg atctattgtc
     5101 aatatttgaa gaaaatcttt tctgagcggc cgctcgagtc tagagggccc gtttaaaccc
     5161 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5221 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5281 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5341 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5401 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5461 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5521 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5581 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5641 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5701 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5761 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5821 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     5881 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5941 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6061 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6121 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6181 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6241 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     6301 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     6361 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     6421 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     6481 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     6541 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     6601 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     6661 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     6721 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6781 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6961 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7201 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
