LOCUS       sequence-156243-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized dead (832A) LbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: WN10151
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgagcaag ctggagaagt
      121 ttacaaactg ctactccctg tctaagaccc tgaggttcaa ggccatccct gtgggcaaga
      181 cccaggagaa catcgacaat aagcggctgc tggtggagga cgagaagaga gccgaggatt
      241 ataagggcgt gaagaagctg ctggatcgct actatctgtc ttttatcaac gacgtgctgc
      301 acagcatcaa gctgaagaat ctgaacaatt acatcagcct gttccggaag aaaaccagaa
      361 ccgagaagga gaataaggag ctggagaacc tggagatcaa tctgcggaag gagatcgcca
      421 aggccttcaa gggcaacgag ggctacaagt ccctgtttaa gaaggatatc atcgagacaa
      481 tcctgccaga gttcctggac gataaggacg agatcgccct ggtgaacagc ttcaatggct
      541 ttaccacagc cttcaccggc ttctttgata acagagagaa tatgttttcc gaggaggcca
      601 agagcacatc catcgccttc aggtgtatca acgagaatct gacccgctac atctctaata
      661 tggacatctt cgagaaggtg gacgccatct ttgataagca cgaggtgcag gagatcaagg
      721 agaagatcct gaacagcgac tatgatgtgg aggatttctt tgagggcgag ttctttaact
      781 ttgtgctgac acaggagggc atcgacgtgt ataacgccat catcggcggc ttcgtgaccg
      841 agagcggcga gaagatcaag ggcctgaacg agtacatcaa cctgtataat cagaa
//
