LOCUS       sequence-156244-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized dead (832A) LbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: WN10151
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..129)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(136..183)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tctagactcg agcggccgcc actgtgctgg atatctgcag aattcttagg catagtcggg
       61 gacatcatat gggtatgcat aatcaggcac gtcgtaggga taagcgtaat ctggaacatc
      121 gtatgggtag gatccctttt tcttttttgc ctggccggcc tttttcgtgg ccgccggcct
      181 tttgtgcttc acgctggtct gggcgtactc cagccactcc ttgttagaga tggcgatctt
      241 caccttatcc agcttctcgt cctcggcctt cttgaactgg ccgatggccc acagcacctt
      301 tctggcgatg ttataggcgc cattggcgtc ggcgttcttt ggcaggatgg cattctcctg
      361 ggcctcatag ttccggctat cgtagaagat gccgtcggag ttcttcacag ggctgatcag
      421 aaaatccacg tcggtgcggc ctgtgatgct gttccgcatc tgcagcatca ggctcatcag
      481 ggccataaag ctagagtaga aggccttgtc ggactgctcg cacagcaggg ctctgatatc
      541 gccctgctga taattgatgc cgtacttgtt gaacagctcc ttataggcgc tggtcaggca
      601 cacctcctcc cagtcgaaca cgttgttctt cttaggattc cggaagattc tgatccggtt
      661 gccgtaggag tacagcttcc acttcttgat gtaatcggcg tctgtgcgag agaagttctt
      721 atagtccagg gcaaactcga acagatcctc ctcgggcacg tacatgatcc tgtcaaagga
      781 gctgatgaac ttcttgg
//
