LOCUS       sequence-156247-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead (908A) AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: WN10150
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac cgccaccatg
      121 acacagttcg agggctttac caacctgtat caggtgagca agacactgcg gtttgagctg
      181 atcccacagg gcaagaccct gaagcacatc caggagcagg gcttcatcga ggaggacaag
      241 gcccgcaatg atcactacaa ggagctgaag cccatcatcg atcggatcta caagacctat
      301 gccgaccagt gcctgcagct ggtgcagctg gattgggaga acctgagcgc cgccatcgac
      361 tcctatagaa aggagaaaac cgaggagaca aggaacgccc tgatcgagga gcaggccaca
      421 tatcgcaatg ccatccacga ctacttcatc ggccggacag acaacctgac cgatgccatc
      481 aataagagac acgccgagat ctacaagggc ctgttcaagg ccgagctgtt taatggcaag
      541 gtgctgaagc agctgggcac cgtgaccaca accgagcacg agaacgccct gctgcggagc
      601 ttcgacaagt ttacaaccta cttctccggc ttttatgaga acaggaagaa cgtgttcagc
      661 gccgaggata tcagcacagc catcccacac cgcatcgtgc aggacaactt ccccaagttt
      721 aaggagaatt gtcacatctt cacacgcctg atcaccgccg tgcccagcct gcgggagcac
      781 tttgagaacg tgaagaaggc catcggcatc ttcgtgagca cctccatcga ggaggtgttt
      841 tccttccctt tttataacca gctgctgaca cagacccaga tcgacctgta taaccagctg
      901 ctgg
//
