LOCUS       sequence-156248-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, activator]; p35S:Cas9 
            D10A/N863A-Hax3CT, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG251
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        243..588
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     531..551
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             685..705
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca acgtctcagg tctctgcaag aattcaagct tggaggtcaa catggtggag
      181 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
      241 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
      301 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
      361 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
      421 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
      481 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      541 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
      601 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
      661 tttacaatta tcgatacaat ggctcctaag aagaagcgga aggttggtgg catggacaag
      721 aagtactcca ttgggctcgc tatcggcaca aacagcgtcg gctgggccgt cattacggac
      781 gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata
      841 aagaagaacc tcattggcgc cctcctgttc gactccggg
//
