LOCUS       sequence-156249-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead (908A) AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: WN10150
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..48)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(49..75)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(82..129)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      401..410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcggggaca tcatatgggt atgcataatc aggcacgtcg tagggataag cgtaatctgg
       61 aacatcgtat gggtaggatc cctttttctt ttttgcctgg ccggcctttt tcgtggccgc
      121 cggccttttg ttgcgcagct cctggatgta ggccagccag tcctgattgg agatgccgtt
      181 ctgcagcttc agatccttgc tctccttcag gtgattcagc agcagctggc ccttcagggc
      241 gatgtggtag gcgccattgg catcggcgtc catgggccac tctgggttct gaaaccggga
      301 gtcgaagcac acgccattca gatcgcgcac ggggctgttg atatagtcct cgcctgtggc
      361 ggcattggag ttccgcatct gcagcacgct gcggatcagg gccaccatgg tgtcgatggc
      421 gtgagaatcg tcattctcca gcagctttgg caggatgttg gagccatccc tgaacacgat
      481 gcccttctcc tccagcaggg cgatcagctc gttggcagga tacaggtccc ggtatctgcc
      541 ggtgaatctg tgattctcga tcactggcac gattctcttg ccggcgatga aaggggtgcc
      601 cttggcgtca aactgtgtct cgttcttctc gaacacgata tcccatgcag gcataaagcc
      661 gggcaggccc ctctggaagg acagatttct gttcatctta aagtgcagga tgaagtcgcc
      721 ggttttcacg tcgtagtgca gaaagtcgaa gccctccagg aagtgcttgc ggctctcgtg
      781 att
//
