LOCUS       sequence-156253-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, 
            nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG252
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(51..153)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        357..702
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     645..665
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             799..819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
       61 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
      121 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc agaagacttc aatcactact
      181 tcgactctag ctgtatataa actcagcttc gttttcttat ctaagcgatg tgggactttt
      241 gaagattgtt ttcaacttag caagaattca agcttggagg tcaacatggt ggagcacgac
      301 actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag ggctattgag
      361 acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc agctatctgt
      421 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      481 aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga tggaccccca
      541 cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa gcaagtggat
      601 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
      661 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctcgag tataagagct
      721 catttttaca acaattacca acaacaacaa acaacaaaca acattacaat tacatttaca
      781 attatcgata caatggctcc taagaagaag cggaaggttg gtggcatgga caagaagtac
      841 tccattgggc tcgctatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac
      901 aaggtgccga gcaaaa
//
