LOCUS       sequence-156256-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, 
            BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG253
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             122..142
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc atcagtcaat
       61 tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagaca gcagggctga
      121 ccccaagaag aagaggaagg tgggttcggg tggcggtagt ggtggcggtg gaagcattgt
      181 tgcccagtta tctcgccctg atccggcgtt ggccgcgttg accaacgacc acctcgtcgc
      241 cttggcctgc ctcggcggac gtcctgcgct ggatgcagtg aaaaagggat tgccgcacgc
      301 gccggccttg atcaaaagaa ccaatcgccg tattcccgaa cgcacatccc atcgcgttgc
      361 cgaccacgcg caagtggttc gcgtgctggg ttttttccag tgccactccc acccagcgca
      421 agcatttgat gacgccatga cgcagttcgg gatgagcagg cacgggttgt tacagctctt
      481 tcgcagagtg ggcgtcaccg aactcgaagc ccgcagtgga acgctccccc cagcctcgca
      541 gcgttgggac cgtatcctcc aggcatcagg gatgaaaagg gccaaaccgt cccctacttc
      601 aactcaaacg ccggatcagg cgtctttgca tgcattcgcc gattcgctgg agcgtgacct
      661 tgatgcgcct agcccaatgc acgagggaga tcagacgcgg gcaagcagcc gtaaacggtc
      721 ccgatcggat cgtgctgtca ccggtccctc cgcacagcaa tcgttcgagg tgcgcgttcc
      781 cgaacagcgc gatgcgttgc atttgcccct cctcagctgg ggtgtaaaac gcccgcgtac
      841 caggatcggc ggcctcctgg atcctggtac gcccatggat g
//
