LOCUS       sequence-156259-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, 
            BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG253
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        356..701
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     644..664
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             798..818
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca gaagacttca atcactactt
      181 cgactctagc tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg
      241 aagattgttt tcaacttagc aagaattcaa gcttggaggt caacatggtg gagcacgaca
      301 ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agatcaaagg gctattgaga
      361 cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
      421 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
      481 aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat ggacccccac
      541 ccacgaggag catcgtggaa aaagaagagg ttccaaccac gtctacaaag caagtggatt
      601 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      661 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctcgagt ataagagctc
      721 atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt acatttacaa
      781 ttatcgatac aatggctcct aagaagaagc ggaaggttgg tggcatggac aagaagtact
      841 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
      901 aggtgccgag caaaaa
//
