LOCUS       sequence-156260-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression MbCpf1 nuclease and Mb crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4495
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgccaccat
      121 gctgttccag gactttaccc acctgtatcc actgtccaag acagtgagat ttgagctgaa
      181 gcccatcgat aggaccctgg agcacatcca cgccaagaac ttcctgtctc aggacgagac
      241 aatggccgat atgcaccaga aggtgaaagt gatcctggac gattaccacc gcgacttcat
      301 cgccgatatg atgggcgagg tgaagctgac caagctggcc gagttctatg acgtgtacct
      361 gaagtttcgg aagaacccaa aggacgatga gctgcagaag cagctgaagg atctgcaggc
      421 cgtgctgaga aaggagatcg tgaagcccat cggcaatggc ggcaagtata aggccggcta
      481 cgacaggctg ttcggcgcca agctgtttaa ggacggcaag gagctgggcg atctggccaa
      541 gttcgtgatc gcacaggagg gagagagctc cccaaagctg gcccacctgg cccacttcga
      601 gaagttttcc acctatttca caggctttca cgataaccgg aagaatatgt attctgacga
      661 ggataagcac accgccatcg cctaccgcct gatccacgag aacctgcccc ggtttatcga
      721 caatctgcag atcctgacca caatcaagca gaagcactct gccctgtacg atcagatcat
      781 caacgagctg accgccagcg gcctggacgt gtctctggcc agccacctgg atggctatca
      841 caagctgctg acacaggagg gcatcaccgc ctacaataca ctgctgggag gaatctccgg
      901 agaggcaggc tctcc
//
