LOCUS       sequence-156261-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression MbCpf1 nuclease and Mb crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4495
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..129)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(136..183)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tctagactcg agcggccgcc actgtgctgg atatctgcag aattcttagg catagtcggg
       61 gacatcatat gggtatgcat aatcaggcac gtcgtaggga taagcgtaat ctggaacatc
      121 gtatgggtag gatccctttt tcttttttgc ctggccggcc tttttcgtgg ccgccggcct
      181 tttcctgttc tgggcgaaat tcagccaggt ctgattgtcg atggccagct tcaccttgtt
      241 cagatcgtcg gagttcttca gctcattcag cagccacagg cccttcaggg cgatgtggta
      301 ggcgccgttg gcatcggcat tctgaggctg tgtatcgtcg gccagggcgc tattaaagaa
      361 cacgccctcg tcgtttgcca cgggggacag gatgaaatcc tcgtcagagg aggcgttgct
      421 gtaccgcagg gccagcaggg ttttcagcag gtacatcaga gacttgtgaa actccttatc
      481 attgttctgg cagatgtcca tgaccaggtt gggctgcttc tcgttgatgt ggtggcgggc
      541 gaacagggac ttcagctcat cattcacgtt gatgcccttg gcggcgccct tattctggtt
      601 ggctgtctta tcgtacacgt accgcttgtc gccgtgggaa cagattgtcc agatctggcg
      661 gctattcttg gccttatcgg taaacttggc gtagtcgatg tgaaactcga agtaatcctt
      721 gtcggcatta tagcagatct tgtcgaactt gccaaagaag gcctggctct gggcgatgtt
      781 ctcgtatctt ggcttcagca gatccacaaa gcctgtctca gg
//
