LOCUS       sequence-156263-       11008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II WT
FEATURES             Location/Qualifiers
     source          1..11008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..930
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1476..1485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1879..1888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7016..7033)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7022..7246
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7292..7720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7379..7398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7589..7610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7729..7749)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7734..8063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7914..8049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7976..7995
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8111..8158
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8177..8551
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8681..8802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8718..8737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8772..8791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8851..8867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8851..8867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8864..8886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8875..8891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8899..8905,8906..8923,8924..8929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8944..8965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9082..9099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9253..9841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9333..9352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10012..10803,10804..10872))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10635..10654
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10873..10977)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
      901 accatggact acaaggacga cgatgacaag gctagcatgc acgggggtgg ccccccctcg
      961 ggggacagcg catgcccgct gcgcaccatc aagagagtcc agttcggagt cctgagtccg
     1021 gatgaactga agcgaatgtc tgtgacggag ggtggcatca aatacccaga gacgactgag
     1081 ggaggccgcc ccaagcttgg ggggctgatg gacccgaggc agggggtgat tgagcggact
     1141 ggccgctgcc aaacatgtgc aggaaacatg acagagtgtc ctggccactt tggccacatt
     1201 gaactggcca agcctgtgtt tcacgtgggc ttcctggtga agacaatgaa agttttgcgc
     1261 tgtgtctgct tcttctgctc caaactgctt gtggactcta acaacccaaa gatcaaggat
     1321 atcctggcta agtccaaggg acagcccaag aagcggctca cacatgtcta cgacctttgc
     1381 aagggcaaaa acatatgcga gggtggggag gagatggaca acaagttcgg tgtggaacaa
     1441 cctgagggtg acgaggatct gaccaaagaa aagggccatg gtggctgtgg gcggtaccag
     1501 cccaggatcc ggcgttctgg cctagagctg tatgcggaat ggaagcacgt taatgaggac
     1561 tctcaggaga agaagatcct gctgagtcca gagcgagtgc atgagatctt caaacgcatc
     1621 tcagatgagg agtgttttgt gctgggcatg gagccccgct atgcacggcc agagtggatg
     1681 attgtcacag tgctgcctgt gcccccgctc tccgtgcggc ctgctgttgt gatgcagggc
     1741 tctgcccgta accaggatga cctgactcac aaactggctg acatcgtgaa gatcaacaat
     1801 cagctgcggc gcaatgagca gaacggcgca gcggcccatg tcattgcaga ggatgtgaag
     1861 ctcctccagt tccatgtggc caccatggtg gacaatgagc tgcctggctt gccccgtgcc
     1921 atgcagaagt ctgggcgtcc cctcaagtcc ctgaagcagc ggttgaaggg caaggaaggc
     1981 cgggtgcgag ggaacctgat gggcaaaaga gtggacttct cggcccgtac tgtcatcacc
     2041 cccgacccca acctctccat tgaccaggtt ggcgtgcccc gctccattgc tgccaacatg
     2101 acctttgcgg agattgtcac ccccttcaac attgacagac ttcaagaact agtgcgcagg
     2161 gggaacagcc agtacccagg cgccaagtac atcatccgag acaatggtga tcgcattgac
     2221 ttgcgtttcc accccaagcc cagtgacctt cacctgcaga ccggctataa ggtggaacgg
     2281 cacatgtgtg atggggacat tgttatcttc aaccggcagc caactctgca caaaatgtcc
     2341 atgatggggc atcgggtccg cattctccca tggtctacct ttcgcttgaa tcttagcgtg
     2401 acaactccgt acaatgcaga ctttgacggg gatgagatga acttgcacct gccacagtct
     2461 ctggagacgc gagcagagat ccaggagctg gccatggttc ctcgcatgat tgtcaccccc
     2521 cagagcaatc ggcctgtcat gggtattgtg caggacacac tcacagcagt gcgcaaattc
     2581 accaagagag acgtcttcct ggagcggggt gaagtgatga acctcctgat gttcctgtcg
     2641 acgtgggatg ggaaggtccc acagccggcc atcctaaagc cccggcccct gtggacaggc
     2701 aagcaaatct tctccctcat catacctggt cacatcaatt gtatccgtac ccacagcacc
     2761 catcccgatg atgaagacag tggcccttac aagcacatct ctcctgggga caccaaggtg
     2821 gtggtggaga atggggagct gatcatgggc atcctgtgta agaagtctct gggcacgtca
     2881 gctggctccc tggtccacat ctcctaccta gagatgggtc atgacatcac tcgcctcttc
     2941 tactccaaca ttcagactgt cattaacaac tggctcctca tcgagggtca tactattggc
     3001 attggggact ccattgctga ttctaagact taccaggaca ttcagaacac tattaagaag
     3061 gccaagcagg acgtaataga ggtcatcgag aaggcacaca acaatgagct ggagcccacc
     3121 ccagggaaca ctctgcggca gacgtttgag aatcaggtga accgcattct taacgatgcc
     3181 cgagacaaga ctggctcctc tgctcagaaa tccctgtctg aatacaacaa cttcaagtct
     3241 atggtcgtgt ccggagctaa aggttccaag attaacatct cccaggtcat tgctgtcgtt
     3301 ggacagcagg acgtcgaggg caagcggatt ccatttggct tcaagcaccg gactctgcct
     3361 cacttcatca aggatgacta cgggcctgag agccgtggct ttgtggagaa ctcctaccta
     3421 gccggcctca cacccactga gttctttttc cacgccatgg ggggtcgtga ggggctcatt
     3481 gacacggctg tcaagactgc tgagactgga tacatccagc ggcggctgat caagtccatg
     3541 gagtcagtga tggtgaagta cgacgcgact gtgcggaact ccatcaacca ggtggtgcag
     3601 ctgcgctacg gcgaagacgg cctggcaggc gagagcgttg agttccagaa cctggctacg
     3661 cttaagcctt ccaacaaggc ttttgagaag aagttccgct ttgattatac caatgagagg
     3721 gccctgcggc gcactctgca ggaggacctg gtgaaggacg tgctgagcaa cgcacacatc
     3781 cagaacgagt tggagcggga atttgagcgg atgcgggagg atcgggaggt gctcagggtc
     3841 atcttcccaa ctggagacag caaggtcgtc ctcccctgta acctgctgcg gatgatctgg
     3901 aatgctcaga aaatcttcca catcaaccca cgccttccct ccgacctgca ccccatcaaa
     3961 gtggtggagg gagtcaagga attgagcaag aagctggtga ttgtgaatgg ggatgaccca
     4021 ctaagtcgac aggcccagga aaatgccacg ctgctcttca acatccacct gcggtccacg
     4081 ttgtgttccc gccgcatggc agaggagttt cggctcagtg gggaggcctt cgactggctg
     4141 cttggggaga ttgagtccaa gttcaaccaa gccattgcgc atcccgggga aatggtgggg
     4201 gctctggctg cgcagtccct tggagaacct gccacccaga tgaccttgaa taccttccac
     4261 tatgctggtg tgtctgccaa gaatgtgacg ctgggtgtgc cccgacttaa ggagctcatc
     4321 aacatttcca agaagccaaa gactccttcg cttactgtct tcctgttggg ccagtccgct
     4381 cgagatgctg agagagccaa ggatattctg tgccgtctgg agcatacaac gttgaggaag
     4441 gtgactgcca acacagccat ctactatgac cccaaccccc agagcacggt ggtggcagag
     4501 gatcaggaat gggtgaatgt ctactatgaa atgcctgact ttgatgtggc ccgaatctcc
     4561 ccctggctgt tgcgggtgga gctggatcgg aagcacatga ctgaccggaa gctcaccatg
     4621 gagcagattg ctgaaaagat caatgctggt tttggtgacg acttgaactg catctttaat
     4681 gatgacaatg cagagaagct ggtgctccgt attcgcatca tgaacagcga tgagaacaag
     4741 atgcaagagg aggaagaggt ggtggacaag atggatgatg atgtcttcct gcgctgcatc
     4801 gagtccaaca tgctgacaga tatgaccctg cagggcatcg agcagatcag caaggtgtac
     4861 atgcacttgc cacagacaga caacaagaag aagatcatca tcacggagga tggggaattc
     4921 aaggccctgc aggagtggat cctggagacg gacggcgtga gcttgatgcg ggtgctgagt
     4981 gagaaggacg tggaccccgt acgcaccacg tccaatgaca ttgtggagat cttcacggtg
     5041 ctgggcattg aagccgtgcg gaaggccctg gagcgggagc tgtaccacgt catctccttt
     5101 gatggctcct atgtcaatta ccgacacttg gctctcttgt gtgataccat gacctgtcgt
     5161 ggccacttga tggccatcac ccgacacgga gtcaaccgcc aggacacagg accactcatg
     5221 aagtgttcct ttgaggaaac ggtggacgtg cttatggaag cagccgcaca cggtgagagt
     5281 gaccccatga agggggtctc tgagaatatc atgctgggcc agctggctcc ggccggcact
     5341 ggctgctttg acctcctgct tgatgcagag aagtgcaagt atggcatgga gatccccacc
     5401 aatatccccg gcctgggggc tgctggaccc accggcatgt tctttggttc agcacccagt
     5461 cccatgggtg gaatctctcc tgccatgaca ccttggaacc agggtgcaac ccctgcctat
     5521 ggcgcctggt cccccagtgt tgggagtgga atgaccccag gggcagccgg cttctctccc
     5581 agtgctgcgt cagatgccag cggcttcagc ccaggttact cccctgcctg gtctcccaca
     5641 ccgggctccc cggggtcccc aggtccctca agcccctaca tcccttcacc aggtggtgcc
     5701 atgtctccca gctactcgcc aacgtcacct gcctacgagc cccgctctcc tgggggctac
     5761 acaccccaga gtccctctta ttcccccact tcaccctcct actcccctac ctctccatcc
     5821 tattctccaa ccagtcccaa ctatagtccc acatcaccca gctattcgcc aacgtcaccc
     5881 agctactcac cgacctctcc cagctactca cccacctctc ccagctactc gcccacctct
     5941 cccagctact cgcccacctc tcccagctac tcacccactt cccctagcta ctcgcccact
     6001 tcccctagct actcgccaac gtctcccagc tactcgccga catctcccag ctactcgcca
     6061 acttcaccca gctattctcc cacttctccc agctactcac ctacctctcc aagctattca
     6121 cccacctccc ccagctactc acccacttcc ccaagttact cacccaccag cccgaactat
     6181 tctccaacca gtcccaatta caccccaaca tcacccagct acagcccgac atcacccagc
     6241 tattcaccta ctagtcccaa ctacacacct accagcccta actacagccc aacctctcca
     6301 agctactctc caacatcacc cagctattcc ccgacctcac caagttactc cccttccagc
     6361 ccacgataca caccacagtc tccaacctat accccaagct cacccagcta cagccccagc
     6421 tcgcccagct acagcccaac ctcacccaag tacaccccaa ccagtccttc ttacagtccc
     6481 agctccccag agtatacccc aacctctccc aagtactcac ctaccagtcc caaatattca
     6541 cccacctctc ccaagtactc gcctaccagt cccacctatt cacccaccac cccaaaatac
     6601 tccccaacat ctcctactta ttccccaacc tctccagtct acaccccaac ctctcccaag
     6661 tactcaccta ctagccccac ttactcgccc acttccccca agtactcgcc caccagcccc
     6721 acctactcgc ccacctcccc caaaggctca acctactctc ccacttcccc tggttactcg
     6781 cccaccagcc ccacctacag tctcacaagc ccggctatca gcccggatga cagtgacgag
     6841 gagaacgcta gctaggggtc cccaggtccc tcaagcccct acatcccttc accaggtggc
     6901 gccatgtagt ctagactcga gcggccgcca ctgtgctgga tatctgcaga attccaccac
     6961 actggactag tggatccgag ctcggtacca agcttaagtt taaaccgctg atcagcctcg
     7021 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7081 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7141 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7201 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7261 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     7321 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7381 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7441 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7501 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7561 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7621 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7681 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7741 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7801 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7861 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7921 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7981 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8041 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     8101 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     8161 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     8221 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     8281 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     8341 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     8401 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     8461 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     8521 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     8581 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8641 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8821 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10981 atttccccga aaagtgccac ctgacgtc
//
