LOCUS       sequence-156266-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression FnCpf1 nuclease and Fn crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4497
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgccaccat
      121 gagcatctac caggagttcg tcaacaagta ttcactgagt aagacactgc ggttcgagct
      181 gatcccacag ggcaagacac tggagaacat caaggcccga ggcctgattc tggacgatga
      241 gaagcgggca aaagactata agaaagccaa gcagatcatt gataaatacc accagttctt
      301 tatcgaggaa attctgagct ccgtgtgcat cagtgaggat ctgctgcaga attactcaga
      361 cgtgtacttc aagctgaaga agagcgacga tgacaacctg cagaaggact tcaagtccgc
      421 caaggacacc atcaagaaac agattagcga gtacatcaag gactccgaaa agtttaaaaa
      481 tctgttcaac cagaatctga tcgatgctaa gaaaggccag gagtccgacc tgatcctgtg
      541 gctgaaacag tctaaggaca atgggattga actgttcaag gctaactccg atatcactga
      601 tattgacgag gcactggaaa tcatcaagag cttcaaggga tggaccacat actttaaagg
      661 cttccacgag aaccgcaaga acgtgtactc cagcaacgac attcctacct ccatcatcta
      721 ccgaatcgtc gatgacaatc tgccaaagtt cctggagaac aaggccaaat atgaatctct
      781 gaaggacaaa gctcccgagg caattaatta cgaacagatc aagaaagatc tggctgagga
      841 actgacattc gatatcgact ataa
//
