LOCUS       sequence-156267-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression FnCpf1 nuclease and Fn crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4497
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..128)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(135..182)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctagactcga gcggccgcca ctgtgctgga tatctgcaga attcttaggc atagtcgggg
       61 acatcatatg ggtatgcata atcaggcacg tcgtagggat aagcgtaatc tggaacatcg
      121 tatgggtagg atcccttttt cttttttgcc tggccggcct ttttcgtggc cgccggcctt
      181 ttgttatttc tattctggac aaactcgaag tattcctcgt tcttaatgac caggttcagc
      241 ttcttcccct cctgattgtt cttgatccgg cccagcagca tcagtccctt cagcccgatg
      301 tggtaggccc cgttggcgtc tgcatcctga ggcatatttt tgggtgcctg tctgctgtcg
      361 aagaagtttc cgttgacatc agccacgggg ctaatcagat agtccagttc tgtcccggtc
      421 tttgagttcc gcatctgcag gattgtattc agcactgagg tcagcttggc gaaaaatttc
      481 ttatcactct cgccacagat ggctgccttg atgcattcgc catgtccata ctcgatagag
      541 tagtctttca gcagcttttc cagctccttg gttgggtaca cctccctagt gtcccaattg
      601 tggtttttat cggaatttcg aaagttgatc aggcgagatc cgaaggaggc aatggtccat
      661 ttccccttag cggccttgtc gccgaagttc ttgtaatcga aggaaaactc gaagtacccc
      721 ttgtccagat tataacagat cttatcgaac ttgctgaaa
//
