LOCUS       sequence-156268-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of NHA-tagged Nematostella vectensis GW182 in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1469
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     681..701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     727..751
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          891..1023
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1006..1025
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1068..1086
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1090..1099
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1096..1161
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1189..1215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6369..6490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6380..6399)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6434..6453
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6676..7131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6763..6782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6973..6994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7248..7605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7248..7439
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7271..7291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7456..7591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7656..8450
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7710..7729)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8320..8339
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8514..8562
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8603..8622)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8722..8744
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8782..8800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8868..8972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8973..9041,9042..9833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9191..9210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10004..10592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10493..10512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
       61 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      121 caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 gcgatcgccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      721 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
      781 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
      841 tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
      901 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
      961 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1021 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac
     1081 tataggctag ccaccatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa
     1141 gctcaatgga aagctgcaaa cggtggatct cctccaagct tgatggctta cccatacgat
     1201 gttccagatt acgctcctgc aggcatgcct aaggatgaca agaaggaatt cctcaagtca
     1261 ttgggcgttg acaccctgtc ctctgaaaac gacatcaaga gagcctataa gaaaatagca
     1321 ctccagtgtc accctgataa gaaccccagc gacacgtccg ccagaaaaca attcgaggag
     1381 gtctctaaag cattcgagta cctcgtccac gacgggatca atgacagcct gaaagattac
     1441 gataaggttt tctttgattt cctccgggac atattgttcg gcagcgatgg tgcttcagac
     1501 ttctttacct atttttgtgg ttccaagcag cacagctgta tctccacaga caccgaccct
     1561 gattttagat atcaggatga cgacgaggag gaggacattg atggggaaca ggagctggaa
     1621 agcctcctgg agatcgtcaa gaaccgccgg aagcgcggga agcccgtacc cgatgagttt
     1681 cacgaggaga aactgcagga gctgaagact cgactgtctc agcacaaaga ggacatgaaa
     1741 aaggatggga gcaagaaaca ggctgttaag aaggggaaag ctgtcagcgt ccctgtggca
     1801 aaacccaagc caaagagcaa aaagcagctc cttgctgaac aacggcggcg ggctaaagaa
     1861 atggagcaga tagccatgga actggaggag aaaagaaaag aagaagaaga aaaggagaag
     1921 caacgcaaac agctggaagt agagaaacag aaacgccttg aggaggagcg catcaggtca
     1981 cagaacgagg agcgaaaacg gcgggagcga gagcaaaaag aacgggagaa gcagcagaaa
     2041 ctggatatgg agaaagagga gcgccgccgg cgccagcagg aggaggagaa gaaaagacga
     2101 gaggaggaag agaagagaaa aaaagtcgag aaagagaagc aggaaaggga gaaggccgca
     2161 aaaaaagctg ccctccaggc tgcgaaggag aaagaagaac aggcgaaggc tgcatctaaa
     2221 aatcagaata ttaaaagaca gcagcaacaa cagcgaactg atcaacagca gtatcctagg
     2281 gaaatacctc ctaggtttca gaaaatgaaa agacagcaac aacagcagca gctgcagact
     2341 ccaaacttgc cacctccagc cccaaccggc tctccggctc agcttaacaa gttccagtca
     2401 aaacctcagc aacagcctgt tggcaagaag aaggatccta accgcttcgc tagcaaggag
     2461 tacgctgacc tcgttgacga ggtggacgat gcgggctgga acaagggatc tcagtgtaaa
     2521 ggctctggat gggaagggac tagcgaggat tgggaggaga acatcgccga aaagtggggc
     2581 gcagataggc agtgggggag caaggaaggg tccagtaacc aactcgttgt ggaccgcaca
     2641 gatggcgata gctggcccac cgtgggcagt aagtctgcta ttggagataa gcccaattct
     2701 tcccttgaga acagtaacag caaaaacagc agtgagaccg tgcctgtggg gacaatcggc
     2761 agcaaacctc caagcagcaa ttggaacagt aacaggaaca gcggaggact cccactgtcc
     2821 gagaattggg atacaacctg tgacgagagc atcgatcgcg ctctggacgc cactgaatct
     2881 aaaggatcag tggggcctgg gggagtcaat tgggttggca tgggtacagg cggattgggc
     2941 gggatcattg gaaccccttt gggttgggag cctagcaaag agaaccgcga tattgataag
     3001 accgcctcta aaagcggctg gtgcggggaa cagaactcta ctgtcgatag ctggaataag
     3061 actccaggta agggcccgaa aacacaggtg ttcggtggat cagcccccac atggggggga
     3121 acaccggcag actcttcttc cgcctgggtg gagactagcg ggactgagac caataaaccc
     3181 aactcctccg gctggaatag caaggtcagc agcggcctga gtcagggaaa ctctagtagc
     3241 atggcctctg tagatagtac cgacaataaa ccaacagaca ataaaccagc tccatcaggc
     3301 tggggcgaag ccggcccatc atgggctgtg caggcttccg ttaccggaac agcatgctgg
     3361 gacaccgcca ctaccacatc ccctaaaaag cctctggatt ccaaagatag caagctgagc
     3421 cacgataagg tgtccgggtg gctcgagaca tccgtgacct ccactagcgt gactgcagat
     3481 tggaacggcg atagcaatgc tgaccaggag gactactacg aagaggcgtg ggacgggtgg
     3541 acaactgcat cctccaagcg aaacaaggcc ccgaagccgg cacccacaaa tgactccaat
     3601 gcatggatgt ccaggagcct taaacagctt ctggacatgg gatttaaaca agaggacgct
     3661 gagaaagcgc tgcgcgccaa tcacatgaac atcgagagcg cgatttctga cctgctgagc
     3721 cttacagcct cccagatcga ggaagcctcc cgagctagtt ctgctaacac cggtgagcct
     3781 gacccggcag aagcctgtag gatgcagcag cagaatacta ctacatctaa gccaaaccga
     3841 aaacaaaggc gcaagaccca gcagcagttg cagcaacagc aggcctctca ggccaagcag
     3901 gagtccaagc aaaccgatat ctacgatgct aatagtcagt cagggtcttc tgactctaca
     3961 gttaaggatc ccaaatctga gtccaataaa gataaagaag gccaggccgc cgagggccag
     4021 agtaataagc cttctaatca gggggctagc gacgggaaag ggaacgcctc aaaaaccaca
     4081 gaaaattctc cacacctctc accgggtaag tccaatgcag gtcagaaacg agccacaact
     4141 ggcctcatcc agacgcctgc attggggacc ccgaccccaa tgagaactcc gatgacacat
     4201 cctttgttgc accagcagtt tctgcatcag gtgcagctcc accagctgag gatggcccag
     4261 ggaggtctcc ttcaacctcc cataaacccc cagcaagtag ctttgttgca gcagatggct
     4321 cagcttcaga tggtccagca tcgcatcgcc cagcagcaac tgacccagaa gcacaacatc
     4381 caggcacaac agcagctgta tcaacagcag caacagattg cactcatgat ggggcagctg
     4441 cagcagcaag tgatgcagca gcacaatcat cagatagccg gttcccggtt ccccagcccc
     4501 ggctttcagc agagcaccct tcctcctcaa agcagttccg cccagcctgg tactccacct
     4561 cagaacgcca agaattccac caaagccact tctactgagc ctagtcaatc aaaacctgag
     4621 gaacccccca aggtgtccac cgcagataag cctgtgacca cagttgccag cggagagcga
     4681 ataactcagc agagccctgc cccacagtcc aggctgaccc agtggaaaca accactcctc
     4741 cctgatcccg agacctctca tggcgaaaca tggggcatgc ccaagattga gcagatgtct
     4801 ctgctgtcag gacctgtagt gagctccagc ctggaacagc agcatggggc tcccaacctt
     4861 tctccacagg caaataggat tgccgatccc gtgagctcac ggtggggcgt tgacgcctct
     4921 cttaagctca gcgccgaccc tccagagttc aagcccggag tcccctggcg ccctcggggg
     4981 gagggggaag aaaagtctgg ttcatctgga agtagtatgg aatcatcccc tcagggcatg
     5041 cctgccagtg gcaagactca gataacagac aacccaaaca agcagccggg ctttccaggc
     5101 gggtttagca gcgctagcca gttcccgtac tctatagcca gcagtccctg gcagaacact
     5161 gaacccacag atagcctgaa caagacccag gcgaacccca gtagtatcag accacctcca
     5221 gggctcaata catctactag tagcggcctc cccgataccc cctctgtcga agagagacct
     5281 gcatggatga aaaatctcat ggatgggggg acccagcaga gctttgccca gggacctaat
     5341 cctgggaact ttcagctggg actgcggttg ggcttctccg ccagcggcaa ttcctggagt
     5401 acccaggacg ggatgtctgt ttccggactg actcctaata gccaaccgtg ggctgcccca
     5461 ggcggaccta gttcttcctc cacaccatcc tctgacccct ctgccgaggc cgccagctcc
     5521 accgccaatg ccgtaacaag tgctgaacag cttaaacctc aggatgaagg gatctcaagt
     5581 tggtccacca accagccaat tccgtctgcg gaggagaaac aacagcagcc acctgtggcc
     5641 caaggaccta ggatgggatc cggtcgcggc ggagccaacc caatgagtac ttggctggtg
     5701 ttgcggaatc tgtccccacg ggcagatccg accgcaatga gggctgtgtg ccagcagtac
     5761 ggcccactcc tcacctttac tctgaacctt aggcacggaa acagcttgat cagatactct
     5821 aataaggatc aagccgcctc agcacggaac aacctcaacg ggatgatggt gaaaggtatg
     5881 cagcttatcg ccgactttgc gacggatagc gatattgggg ggttttttga gcagacaccc
     5941 gactggtcca acaacccaat gccacccaac cctagcttcg gcaacccttg gtccttcgga
     6001 acagttgatc ctaatcgacc tctcggatcc actcaaggac tggagaaaac gccgcaaggc
     6061 cccccacctc aaatgcctgc aatggttccg ccgggcatgc aatggggaag tagttctcag
     6121 ctccccgctc aactctgggg caactcccat cctggtgccg ccacaggctt cccgtctcag
     6181 gtgccatcca tgtggtcttt cagcggagga gcccctcgcg atgtcgaacc ccaatccgga
     6241 acaaatgaga atgggttggt ctctccatcc atgactacgt ttctgcctcc cgggttgctg
     6301 aacggtggtg agagtgtcta aggcggccgc ttccctttag tgagggttaa tgcttcgagc
     6361 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6421 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6481 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     6541 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat
     6601 ccgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6661 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6721 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6781 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6841 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6901 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6961 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7021 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7081 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     7141 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     7201 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     7261 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7321 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7381 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7441 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7561 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct
     7621 gacacaacag tctcgaactt aaggctagag ccaccatgat tgaacaagat ggattgcacg
     7681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     8041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     8101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     8161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     8221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     8281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa
     8521 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     8581 tccgcgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8641 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8701 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8761 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8821 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8881 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8941 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9001 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9061 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9121 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9181 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9241 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9301 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9361 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9421 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9481 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9541 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9601 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9661 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9721 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9781 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9841 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10141 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10621 tcctggcctt ttgctggcct tttgctcaca tggctcgaca gatc
//
