LOCUS       sequence-156269-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (GFP)
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..120
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        148..658
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     577..595
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtttagtgaa ccgtcagatc gcctggagaa ttggctagcg aattcgtcga caccggttcc
       61 ggagccacga acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct
      121 aggctgcaga cgcgttgtac aggatccggg gttggggttg cgccttttcc aaggcagccc
      181 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
      241 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
      301 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
      361 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
      421 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
      481 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
      541 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
      601 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccagca
      661 attcacccgc gcgcggccac catggagagc gacgagagcg gcctgcccgc catggagatc
      721 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
      781 ggcacccccg agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
      841 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
      901 cccagcggct acgagaaccc cttcctgcac gccatcaaca acgg
//
