LOCUS       sequence-156271-       10577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of HA-tagged Nematostella vectensis GW182 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1468
FEATURES             Location/Qualifiers
     source          1..10577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     681..701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     727..751
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          891..1023
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1006..1025
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1068..1086
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1090..1099
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1102..1128
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6282..6403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6293..6312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6347..6366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6589..7044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6676..6695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6886..6907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7161..7518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7161..7352
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7184..7204)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7369..7504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7569..8363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7623..7642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8233..8252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8427..8475
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8516..8535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8635..8657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8695..8713)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8781..8885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8886..8954,8955..9746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9104..9123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9917..10505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10406..10425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
       61 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      121 caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 gcgatcgccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      721 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
      781 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
      841 tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
      901 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
      961 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1021 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac
     1081 tataggctag ccaccatggc ttacccatac gatgttccag attacgctcc tgcaggcatg
     1141 cctaaggatg acaagaagga attcctcaag tcattgggcg ttgacaccct gtcctctgaa
     1201 aacgacatca agagagccta taagaaaata gcactccagt gtcaccctga taagaacccc
     1261 agcgacacgt ccgccagaaa acaattcgag gaggtctcta aagcattcga gtacctcgtc
     1321 cacgacggga tcaatgacag cctgaaagat tacgataagg ttttctttga tttcctccgg
     1381 gacatattgt tcggcagcga tggtgcttca gacttcttta cctatttttg tggttccaag
     1441 cagcacagct gtatctccac agacaccgac cctgatttta gatatcagga tgacgacgag
     1501 gaggaggaca ttgatgggga acaggagctg gaaagcctcc tggagatcgt caagaaccgc
     1561 cggaagcgcg ggaagcccgt acccgatgag tttcacgagg agaaactgca ggagctgaag
     1621 actcgactgt ctcagcacaa agaggacatg aaaaaggatg ggagcaagaa acaggctgtt
     1681 aagaagggga aagctgtcag cgtccctgtg gcaaaaccca agccaaagag caaaaagcag
     1741 ctccttgctg aacaacggcg gcgggctaaa gaaatggagc agatagccat ggaactggag
     1801 gagaaaagaa aagaagaaga agaaaaggag aagcaacgca aacagctgga agtagagaaa
     1861 cagaaacgcc ttgaggagga gcgcatcagg tcacagaacg aggagcgaaa acggcgggag
     1921 cgagagcaaa aagaacggga gaagcagcag aaactggata tggagaaaga ggagcgccgc
     1981 cggcgccagc aggaggagga gaagaaaaga cgagaggagg aagagaagag aaaaaaagtc
     2041 gagaaagaga agcaggaaag ggagaaggcc gcaaaaaaag ctgccctcca ggctgcgaag
     2101 gagaaagaag aacaggcgaa ggctgcatct aaaaatcaga atattaaaag acagcagcaa
     2161 caacagcgaa ctgatcaaca gcagtatcct agggaaatac ctcctaggtt tcagaaaatg
     2221 aaaagacagc aacaacagca gcagctgcag actccaaact tgccacctcc agccccaacc
     2281 ggctctccgg ctcagcttaa caagttccag tcaaaacctc agcaacagcc tgttggcaag
     2341 aagaaggatc ctaaccgctt cgctagcaag gagtacgctg acctcgttga cgaggtggac
     2401 gatgcgggct ggaacaaggg atctcagtgt aaaggctctg gatgggaagg gactagcgag
     2461 gattgggagg agaacatcgc cgaaaagtgg ggcgcagata ggcagtgggg gagcaaggaa
     2521 gggtccagta accaactcgt tgtggaccgc acagatggcg atagctggcc caccgtgggc
     2581 agtaagtctg ctattggaga taagcccaat tcttcccttg agaacagtaa cagcaaaaac
     2641 agcagtgaga ccgtgcctgt ggggacaatc ggcagcaaac ctccaagcag caattggaac
     2701 agtaacagga acagcggagg actcccactg tccgagaatt gggatacaac ctgtgacgag
     2761 agcatcgatc gcgctctgga cgccactgaa tctaaaggat cagtggggcc tgggggagtc
     2821 aattgggttg gcatgggtac aggcggattg ggcgggatca ttggaacccc tttgggttgg
     2881 gagcctagca aagagaaccg cgatattgat aagaccgcct ctaaaagcgg ctggtgcggg
     2941 gaacagaact ctactgtcga tagctggaat aagactccag gtaagggccc gaaaacacag
     3001 gtgttcggtg gatcagcccc cacatggggg ggaacaccgg cagactcttc ttccgcctgg
     3061 gtggagacta gcgggactga gaccaataaa cccaactcct ccggctggaa tagcaaggtc
     3121 agcagcggcc tgagtcaggg aaactctagt agcatggcct ctgtagatag taccgacaat
     3181 aaaccaacag acaataaacc agctccatca ggctggggcg aagccggccc atcatgggct
     3241 gtgcaggctt ccgttaccgg aacagcatgc tgggacaccg ccactaccac atcccctaaa
     3301 aagcctctgg attccaaaga tagcaagctg agccacgata aggtgtccgg gtggctcgag
     3361 acatccgtga cctccactag cgtgactgca gattggaacg gcgatagcaa tgctgaccag
     3421 gaggactact acgaagaggc gtgggacggg tggacaactg catcctccaa gcgaaacaag
     3481 gccccgaagc cggcacccac aaatgactcc aatgcatgga tgtccaggag ccttaaacag
     3541 cttctggaca tgggatttaa acaagaggac gctgagaaag cgctgcgcgc caatcacatg
     3601 aacatcgaga gcgcgatttc tgacctgctg agccttacag cctcccagat cgaggaagcc
     3661 tcccgagcta gttctgctaa caccggtgag cctgacccgg cagaagcctg taggatgcag
     3721 cagcagaata ctactacatc taagccaaac cgaaaacaaa ggcgcaagac ccagcagcag
     3781 ttgcagcaac agcaggcctc tcaggccaag caggagtcca agcaaaccga tatctacgat
     3841 gctaatagtc agtcagggtc ttctgactct acagttaagg atcccaaatc tgagtccaat
     3901 aaagataaag aaggccaggc cgccgagggc cagagtaata agccttctaa tcagggggct
     3961 agcgacggga aagggaacgc ctcaaaaacc acagaaaatt ctccacacct ctcaccgggt
     4021 aagtccaatg caggtcagaa acgagccaca actggcctca tccagacgcc tgcattgggg
     4081 accccgaccc caatgagaac tccgatgaca catcctttgt tgcaccagca gtttctgcat
     4141 caggtgcagc tccaccagct gaggatggcc cagggaggtc tccttcaacc tcccataaac
     4201 ccccagcaag tagctttgtt gcagcagatg gctcagcttc agatggtcca gcatcgcatc
     4261 gcccagcagc aactgaccca gaagcacaac atccaggcac aacagcagct gtatcaacag
     4321 cagcaacaga ttgcactcat gatggggcag ctgcagcagc aagtgatgca gcagcacaat
     4381 catcagatag ccggttcccg gttccccagc cccggctttc agcagagcac ccttcctcct
     4441 caaagcagtt ccgcccagcc tggtactcca cctcagaacg ccaagaattc caccaaagcc
     4501 acttctactg agcctagtca atcaaaacct gaggaacccc ccaaggtgtc caccgcagat
     4561 aagcctgtga ccacagttgc cagcggagag cgaataactc agcagagccc tgccccacag
     4621 tccaggctga cccagtggaa acaaccactc ctccctgatc ccgagacctc tcatggcgaa
     4681 acatggggca tgcccaagat tgagcagatg tctctgctgt caggacctgt agtgagctcc
     4741 agcctggaac agcagcatgg ggctcccaac ctttctccac aggcaaatag gattgccgat
     4801 cccgtgagct cacggtgggg cgttgacgcc tctcttaagc tcagcgccga ccctccagag
     4861 ttcaagcccg gagtcccctg gcgccctcgg ggggaggggg aagaaaagtc tggttcatct
     4921 ggaagtagta tggaatcatc ccctcagggc atgcctgcca gtggcaagac tcagataaca
     4981 gacaacccaa acaagcagcc gggctttcca ggcgggttta gcagcgctag ccagttcccg
     5041 tactctatag ccagcagtcc ctggcagaac actgaaccca cagatagcct gaacaagacc
     5101 caggcgaacc ccagtagtat cagaccacct ccagggctca atacatctac tagtagcggc
     5161 ctccccgata ccccctctgt cgaagagaga cctgcatgga tgaaaaatct catggatggg
     5221 gggacccagc agagctttgc ccagggacct aatcctggga actttcagct gggactgcgg
     5281 ttgggcttct ccgccagcgg caattcctgg agtacccagg acgggatgtc tgtttccgga
     5341 ctgactccta atagccaacc gtgggctgcc ccaggcggac ctagttcttc ctccacacca
     5401 tcctctgacc cctctgccga ggccgccagc tccaccgcca atgccgtaac aagtgctgaa
     5461 cagcttaaac ctcaggatga agggatctca agttggtcca ccaaccagcc aattccgtct
     5521 gcggaggaga aacaacagca gccacctgtg gcccaaggac ctaggatggg atccggtcgc
     5581 ggcggagcca acccaatgag tacttggctg gtgttgcgga atctgtcccc acgggcagat
     5641 ccgaccgcaa tgagggctgt gtgccagcag tacggcccac tcctcacctt tactctgaac
     5701 cttaggcacg gaaacagctt gatcagatac tctaataagg atcaagccgc ctcagcacgg
     5761 aacaacctca acgggatgat ggtgaaaggt atgcagctta tcgccgactt tgcgacggat
     5821 agcgatattg gggggttttt tgagcagaca cccgactggt ccaacaaccc aatgccaccc
     5881 aaccctagct tcggcaaccc ttggtccttc ggaacagttg atcctaatcg acctctcgga
     5941 tccactcaag gactggagaa aacgccgcaa ggccccccac ctcaaatgcc tgcaatggtt
     6001 ccgccgggca tgcaatgggg aagtagttct cagctccccg ctcaactctg gggcaactcc
     6061 catcctggtg ccgccacagg cttcccgtct caggtgccat ccatgtggtc tttcagcgga
     6121 ggagcccctc gcgatgtcga accccaatcc ggaacaaatg agaatgggtt ggtctctcca
     6181 tccatgacta cgtttctgcc tcccgggttg ctgaacggtg gtgagagtgt ctaaggcggc
     6241 cgcttccctt tagtgagggt taatgcttcg agcagacatg ataagataca ttgatgagtt
     6301 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6361 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6421 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     6481 ctacaaatgt ggtaaaatcc gataaggatc gatccgggct ggcgtaatag cgaagaggcc
     6541 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     6601 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6661 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6721 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6781 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6841 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6901 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6961 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7021 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     7081 tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga
     7141 ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     7201 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7261 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7321 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7441 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7501 ggcctaggct tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta
     7561 gagccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7621 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7681 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7741 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7801 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7861 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7921 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7981 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8041 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     8101 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8161 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8221 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8281 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8341 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     8401 gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg
     8461 ttggtttttt gtgtgaatcg atagcgataa ggatccgcgt atggtgcact ctcagtacaa
     8521 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8581 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8641 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8701 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10561 acatggctcg acagatc
//
