LOCUS       sequence-156285-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 expressed behind an ATc-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pTet01
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..58
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(133..160)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(176..247)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(295..327)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
ORIGIN
        1 aacgcatgaa ctctttgata acgtcttcgg aggaagccat ctagtatttc tcctctttct
       61 agtagtgctc agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact
      121 ctagtatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc tagtagagag
      181 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      241 ttatttgatg cctggctcta gtagtgatct acactagcac tatcagtgtt attaagctac
      301 taaagcgtag ttttcgtcgt ttgcagcgga cccactttca catttaagtt gtttttctaa
      361 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa
      421 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc
      481 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac
      541 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa
      601 ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc
      661 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc
      721 ttgccagctt tccccttcta aag
//
