LOCUS       sequence-156290-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Lenstra TL, Coulon A, Chow CC, Larson DR
  TITLE     Single-Molecule Imaging Reveals a Switch between Spurious and 
            Functional ncRNA Transcription.
  JOURNAL   Mol Cell. 2015 Nov 19;60(4):597-610. doi: 
            10.1016/j.molcel.2015.09.028. Epub 2015 Nov 5.
  PUBMED    26549684
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09.028"; journalName: "Mol Cell"; date: 
            "2015-11-19- 19"; volume: "60"; issue: "4"; pages: "597-610"
COMMENT     Sequence Label: pTL032
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..24
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6..24
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(75..108)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(171..368)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgcgcaatta accctcacta aagggaacaa aagctgggta ccgcataggc cactagtgga
       61 tctgatatca cctaataact tcgtatagca tacattatac gaagttatat taagggttct
      121 cgagagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg
      181 accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct
      241 gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga
      301 acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta
      361 ttgtcagtac tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
      421 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
      481 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
      541 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
      601 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
      661 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
      721 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
      781 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
      841 ctgaatcagg atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga
      901 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
      961 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1021 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1081 ctgattgccc
//
