LOCUS       sequence-156295-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Lenstra TL, Coulon A, Chow CC, Larson DR
  TITLE     Single-Molecule Imaging Reveals a Switch between Spurious and 
            Functional ncRNA Transcription.
  JOURNAL   Mol Cell. 2015 Nov 19;60(4):597-610. doi: 
            10.1016/j.molcel.2015.09.028. Epub 2015 Nov 5.
  PUBMED    26549684
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09.028"; journalName: "Mol Cell"; date: 
            "2015-11-19- 19"; volume: "60"; issue: "4"; pages: "597-610"
COMMENT     Sequence Label: pTL032
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(102..135)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgcgcacgt caagactgtc aaggagggta ttctgggcct ccatgtcgct ggccgggtga
       61 cccggcgggg acgaggcaag ctaaacagat ctctagacct aataacttcg tatagcatac
      121 attatacgaa gttatattaa gggttgtcga cctgcagcgt acaattcctg cagccctaca
      181 aagtggagcg aggagatcct aaggtaccta attgcctaga aaggagcaga cgatatggcg
      241 tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct ggctgcagta
      301 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag acgatatggc
      361 gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc tggctgcagt
      421 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
      481 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
      541 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
      601 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
      661 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
      721 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
      781 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
      841 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
      901 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
      961 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
     1021 gcagtattcc cgggttcatt agatctcgag aacgggg
//
