LOCUS       sequence-156314-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Gsdmd-NT 4A mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Liu X, Zhang Z, Ruan J, Pan Y, Magupalli VG, Wu H, Lieberman J
  TITLE     Inflammasome-activated gasdermin D causes pyroptosis by forming 
            membrane pores.
  JOURNAL   Nature. 2016 Jul 6;535(7610):153-8. doi: 10.1038/nature18629.
  PUBMED    27383986
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-07-6- 6"; volume: "535"; issue: "7610"; pages:
            "153-8"
COMMENT     Sequence Label: Flag-Gsdmd-NT 4A
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..83
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ttcagatcga tctaccatgg
       61 actacaaaga cgatgacgac aagcttgcgg ccgcaatgcc atcggccttt gagaaagtgg
      121 tcaagaatgt gatcaaggag gtaagcggca gcagaggcga tctcattccg gtggacagcc
      181 tgcggaactc caccagcttc aggccctact gccttctgaa caggaaattt tcaagctcaa
      241 ggttctggaa accccgttat tcatgtgtca acctgtcaat caaggacatc ctggagccca
      301 gtgctccaga accagaaccg gagtgttttg gctccttcaa agtctctgat gtcgtcgatg
      361 ggaacattca gggcagagtg atgttgtcag gcatgggaga agggaaaatt tctggtgggg
      421 ctgcagtgtc tgacagttcc agtgcctcca tgaatgtgtg tatactgcgt gtgactcaga
      481 agacctggga gaccatgcag catgaagcgc accttcagca gcctgagaac gcaatcctgc
      541 aacagcttgc gagtgctggg gatgacctgt ttgtggtgac cgaggtgctg cagacaaagg
      601 aggaagtgca gatcactgag gtccacagcc aagagggctc aggccagttt acgctgcctg
      661 gagctttatg cttgaagggt gaaggcaagg gccaccaaag ccggaagaag atggtgacca
      721 ttcctgcagg cagcatcctg gcattccgag tggcccaact gcttattggc tctaaatggg
      781 atatccttct cgtctcagat gagaaacaga ggacctttga gccctcctca ggtgacagaa
      841 aagcagtggg ccagaggcac catggcctca atgtgcttgc tgcgctttgt tccatcggaa
      901 agcagctcag tctcctgtca gattagtcta gaggatcccg ggtggcatcc ctgtgacccc
      961 tcccca
//
