LOCUS       sequence-156319-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Gsdmd-CT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Liu X, Zhang Z, Ruan J, Pan Y, Magupalli VG, Wu H, Lieberman J
  TITLE     Inflammasome-activated gasdermin D causes pyroptosis by forming 
            membrane pores.
  JOURNAL   Nature. 2016 Jul 6;535(7610):153-8. doi: 10.1038/nature18629.
  PUBMED    27383986
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-07-6- 6"; volume: "535"; issue: "7610"; pages:
            "153-8"
COMMENT     Sequence Label: Flag-Gsdmd-CT
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(845..868)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(912..963)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(941..961)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgggatcc ctaacaaggt ttctggccta gacttgacaa taggaacagg gaggcataga
      241 gcgcacttgt ggggatcaga gacgttggtt cccagtgcac ctggggggat tctacctgca
      301 gccttaggcc acattcttct agcaagaccc aggtgggatt cttttcatcc cagcagtccc
      361 caaggagcac ggtgggcagg gacacagaac tctgctcctg ccacggggtg ctctgttcca
      421 agacgtgctt caccaactcc agctgctttg acagcaccgt tgtctccaga gccttagcta
      481 gcagctgctg ctgggtttca ctcagcacag ccagtgctcc cagcaggtag aagatagggg
      541 ctgcgagttc cggcaccagt tctccagagt caagcaccag acactcaagg atgcagccag
      601 ctgggccgtc cagaggctcc acctggccgc cactgcacag gccctgccct agtgaggcct
      661 ctaaggcttc catgctgggc tggtcctgta aaatctttcc gatgttcacc agtatctgtt
      721 gtctcaactc catttccaag ctctccagtt ctgaggagca agccttcacc tcagcataca
      781 ggccctggaa gtctgccgcc tcaattaatt cctcctcatc aatccctgaa ttcgcggccg
      841 caagcttgtc gtcatcgtct ttgtagtcca tggtagatcg atctgaatta attctgacgg
      901 ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      961 gtcaacgggg cggggttatt acgacatttt ggaa
//
