LOCUS       sequence-156321-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Taniguchi N, Carames B, Kawakami Y, Amendt BA, Komiya S, Lotz M
  TITLE     Chromatin protein HMGB2 regulates articular cartilage surface 
            maintenance via beta-catenin pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 29. 106(39):16817-22.
  PUBMED    19805379
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 29. 106(39):16817-22."
COMMENT     Sequence Label: pcDNA3.1 Flag hHMGB1
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             263..286
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctccac aattcgggcg gcggctgctg ctgcctgtgg cccgggcggc tgggagaagc
      121 cgagtgttgg tgagtgacgc ggcggaggtg tagtttgacg cggtgtgtta cgtgggggag
      181 agaataaaac tccagcgaga tccgggccgc gaacgaaagc agtgacggag gagcttgtac
      241 caccggtgac tagatgacca tggactacaa ggacgacgat gacaagctgc aggaattcat
      301 gggcaaagga gatcctaaga agccgagagg caaaatgtca tcatatgcat tttttgtgca
      361 aacttgtcgg gaggagcata agaagaagca cccagatgct tcagtcaact tctcagagtt
      421 ttctaagaag tgctcagaga ggtggaagac catgtctgct aaagagaaag gaaaatttga
      481 agatatggca aaggcggaca aggcccgtta tgaaagagaa atgaaaacct atatccctcc
      541 caaaggggag acaaaaaaga agttcaagga tcccaatgca cccaagaggc ctccttcggc
      601 cttcttcctc ttctgctctg agtatcgccc aaaaatcaaa ggagaacatc ctggcctgtc
      661 cattggtgat gttgcgaaga aactgggaga gatgtggaat aacactgctg cagatgacaa
      721 gcagccttat gaaaagaagg ctgcgaagct gaaggaaaaa tacgaaaagg atattgctgc
      781 atatcgagct aaaggaaagc ctgatgcagc aaaaaaggga gttgtcaagg ctgaaaaaag
      841 caagaaaaag aaggaagagg aggaagatga ggaagatgaa gaggatgagg aggaggagga
      901 agatgaagaa gatgaagatg a
//
