LOCUS       sequence-156324-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT,
            Yeh JR, Joung JK
  TITLE     Efficient genome editing in zebrafish using a CRISPR-Cas system.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
  PUBMED    23360964
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501."
COMMENT     Sequence Label: MLM3613
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag
       61 agccgccacc atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg
      121 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
      181 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
      241 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa
      301 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt
      361 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc
      421 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca
      481 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc
      541 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga
      601 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga
      661 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag
      721 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt
      781 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat c
//
