LOCUS       sequence-156337-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 
            expression. Contains NsiI cloning site for U6-sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Dow LE, Fisher J, O'Rourke KP, Muley A, Kastenhuber ER, Livshits G, 
            Tschaharganeh DF, Socci ND, Lowe SW
  TITLE     Inducible in vivo genome editing with CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 
            2015 Feb 18.
  PUBMED    25690852
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3155"; 
            journalName: "Nat Biotechnol"; date: "2015-04"; volume: "33"; issue:
            "4"; pages: "390-4"
COMMENT     Sequence Label: c3GIC9
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(75..77,78..80,81..794)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(120..141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(381..400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     728..749
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa agtcgagctt
       61 gcgttggatc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg
      181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      241 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      481 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      541 acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca
      601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      661 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      721 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      781 agctgtacaa gtaatgaatt ttccgccccc cccccctaac gttactggcc gaagccgctt
      841 ggaataaggc cggtgtgcgt ttgtctatat gtta
//
