LOCUS       sequence-156343-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector containing doxycycline inducible human miR-200c. 
            Puromycin selectable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: pINDUCER13 miR-200c-141
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(95..113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(105..152)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(222..240,241..263,264..282,283..305,306..324,325..347,
                     348..366,367..389,390..408,409..431,432..450,451..473,
                     474..492)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        525..563
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..584
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      654..663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(726..744)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg
       61 gaacggcact ggtcaacttg gccatggtgg ccctcctata gtgagtcgta ttatactatg
      121 ccgatatact atgccgatga ttaattgtca acacgtgctg caggtccgag gttctaggct
      181 agcttaaagg aaccaattca gtcgactgga tcctttacca ctccctatca gtgatagaga
      241 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      301 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      361 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      421 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      481 cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt
      541 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
      601 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctaccg gtcgccacca
      661 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
      721 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      781 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
      841 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      901 gcagtgaaaa ctctcttca
//
