LOCUS       sequence-156345-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing SMAD4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SMAD4_p.D537Y
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
       61 tggcatggac aatatgtcta ttacgaatac accaacaagt aatgatgcct gtctgagcat
      121 tgtgcatagt ttgatgtgcc atagacaagg tggagagagt gaaacatttg caaaaagagc
      181 aattgaaagt ttggtaaaga agctgaagga gaaaaaagat gaattggatt ctttaataac
      241 agctataact acaaatggag ctcatcctag taaatgtgtt accatacaga gaacattgga
      301 tgggaggctt caggtggctg gtcggaaagg atttcctcat gtgatctatg cccgtctctg
      361 gaggtggcct gatcttcaca aaaatgaact aaaacatgtt aaatattgtc agtatgcgtt
      421 tgacttaaaa tgtgatagtg tctgtgtgaa tccatatcac tacgaacgag ttgtatcacc
      481 tggaattgat ctctcaggat taacactgca gagtaatgct ccatcaagta tgatggtgaa
      541 ggatgaatat gtgcatgact ttgagggaca gccatcgttg tccactgaag gacattcaat
      601 tcaaaccatc cagcatccac caagtaatcg tgcatcgaca gagacataca gcaccccagc
      661 tctgttagcc ccatctgagt ctaatgctac cagcactgcc aactttccca acattcctgt
      721 ggcttccaca agtcagcctg ccagtatact ggggggcagc catagtgaag gactgttgca
      781 gatagcatca gggcctcagc caggacagca gcagaatgga tttactggtc agccagctac
      841 ttaccatcat aacagcacta ccacctggac tggaagtagg actgcaccat acacacctaa
      901 tttgcctcac caccaaaacg gccatcttca gcaccacccg cctatgc
//
