LOCUS       sequence-156349-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing SMAD4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SMAD4_p.K519N
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc
       61 atggacaata tgtctattac gaatacacca acaagtaatg atgcctgtct gagcattgtg
      121 catagtttga tgtgccatag acaaggtgga gagagtgaaa catttgcaaa aagagcaatt
      181 gaaagtttgg taaagaagct gaaggagaaa aaagatgaat tggattcttt aataacagct
      241 ataactacaa atggagctca tcctagtaaa tgtgttacca tacagagaac attggatggg
      301 aggcttcagg tggctggtcg gaaaggattt cctcatgtga tctatgcccg tctctggagg
      361 tggcctgatc ttcacaaaaa tgaactaaaa catgttaaat attgtcagta tgcgtttgac
      421 ttaaaatgtg atagtgtctg tgtgaatcca tatcactacg aacgagttgt atcacctgga
      481 attgatctct caggattaac actgcagagt aatgctccat caagtatgat ggtgaaggat
      541 gaatatgtgc atgactttga gggacagcca tcgttgtcca ctgaaggaca ttcaattcaa
      601 accatccagc atccaccaag taatcgtgca tcgacagaga catacagcac cccagctctg
      661 ttagccccat ctgagtctaa tgctaccagc actgccaact ttcccaacat tcctgtggct
      721 tccacaagtc agcctgccag tatactgggg ggcagccata gtgaaggact gttgcagata
      781 gcatcagggc ctcagccagg acagcagcag aatggattta ctggtcagcc agctacttac
      841 catcataaca gcactaccac ctggactgga agtaggactg caccatacac acctaatttg
      901 cctcaccacc aaaacggcca tcttcagcac cacccgccta tgccgcccca tcccggacat
      961 tactggcctg ttcacaatga gcttgcattc cagcctccca tttcca
//
